Document Detail


Structural and molecular characterization of Shigella boydii type 16 O antigen.
MedLine Citation:
PMID:  16859842     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Shigella is a well-known human pathogen causing dysentery and their typing is solely based on the O antigens. We investigated the chemical structure and gene cluster of Shigella boydii type 16 O antigen. As judged by sugar and methylation analyses along with NMR spectroscopy data, the O antigen has an O-acetylated branched pentasaccharide repeating O unit, which consists of two D-mannose residues (D-Man), one residue each of d-glucuronic acid (D-GlcA), N-acetylglucosamine (D-GlcNAc) and D-galactose (D-Gal), and the structure of the O unit was established. The O antigen gene cluster of S. boydii type 16 was identified and shown to contain putative genes for the synthesis of GDP-D-Man, genes encoding sugar transferases, O unit flippase (Wzx) and O antigen polymerase (Wzy) as expected. The function of the wzy gene was characterized by mutation test. Genes specific to S. boydii type 16 O antigen gene cluster were identified by screening 186 Escherichia coli and Shigella type strains, and can be used to develop PCR assays for detection of type 16 strains.
Authors:
Bin Liu; Sof'ya N Senchenkova; Lu Feng; Andrei V Perepelov; Tianzheng Xu; Sergei D Shevelev; Yajuan Zhu; Alexander S Shashkov; Ming Zou; Yuriy A Knirel; Lei Wang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-06-08
Journal Detail:
Title:  Gene     Volume:  380     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-04     Completed Date:  2006-10-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  46-53     Citation Subset:  IM    
Affiliation:
TEDA School of Biological Sciences and Biotechnology, Nankai University, 23# HongDa Street, TEDA, Tianjin 300457, P.R. China.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ371800
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Carbohydrate Sequence
DNA Primers / genetics
DNA, Bacterial / genetics
Genes, Bacterial
Humans
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Multigene Family
O Antigens / chemistry*,  classification,  genetics*,  metabolism
Oligosaccharides / chemistry,  genetics
Polymerase Chain Reaction
Shigella boydii / genetics*,  immunology*,  metabolism,  pathogenicity
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Bacterial; 0/O Antigens; 0/Oligosaccharides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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