| Structural isoforms of the circadian neuropeptide PDF expressed in the optic lobes of the cricket Gryllus bimaculatus: immunocytochemical evidence from specific monoclonal antibodies. | |
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MedLine Citation:
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PMID: 16998911 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pigment-dispersing factor (PDF) is an 18-mer peptide that acts as a principal neurotransmitter of the insect circadian clock. Our previous study, utilizing anti-Uca beta-PDH polyclonal antibody (pAb) to immunolabel the optic lobe of the cricket Gryllus bimaculatus, suggested the existence of an alternative PDF-like peptide in the outer cells of the first neuropile, or lamina (La), which were much less immunoreactive than the inner cells of the second neuropile, the medulla (Me). To obtain structural information about such a PDF-like peptide, we prepared 10 anti-Gryllus PDF monoclonal (mAb) and pAb antibodies and analyzed their detailed epitope specificities. The PDFMe and PDFLa inner cells and their axonal projections were clearly immunoreactive to all these antibodies, revealing the widespread immunocytochemical organization of the PDF system in the optic lobe, as seen previously with anti-Uca beta-PDH pAb and anti-Gryllus PDF mAb, the epitope structures of which were also clarified in this study. The lamina outer cells, which we found lacked a target pdf mRNA, displayed specific immunoreactivities, indicating that the cells contain a distinct PDF-like peptide possessing both N- and C-terminal structures. These cells were not immunolabeled by some other monoclonal antibodies, however, implying that the PDFLa outer cells have a PDF isoform peptide devoid of Asn at positions 6 and 16. This isoform was also identified in a varicose arborization in the lamina. These results suggest not only the structure of the peptide, but also the possibility of additional functions of this novel PDF isoform. |
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Authors:
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Takeshi Honda; Ayami Matsushima; Kazunori Sumida; Yoshiro Chuman; Kazuyasu Sakaguchi; Hitoshi Onoue; Ian A Meinertzhagen; Yasuyuki Shimohigashi; Miki Shimohigashi |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of comparative neurology Volume: 499 ISSN: 0021-9967 ISO Abbreviation: J. Comp. Neurol. Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-10-02 Completed Date: 2006-11-24 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0406041 Medline TA: J Comp Neurol Country: United States |
Other Details:
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Languages: eng Pagination: 404-21 Citation Subset: IM |
Affiliation:
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Laboratory of Structure-Function Biochemistry, Department of Chemistry, Faculty and Graduate School of Sciences, Kyushu University, Fukuoka 812-8581, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / immunology Antibody Specificity / immunology Axons / metabolism, ultrastructure Brain / cytology, metabolism* Circadian Rhythm / physiology Drosophila Proteins / chemistry, immunology, metabolism Epitope Mapping / methods Epitopes / chemistry, immunology Gryllidae / anatomy & histology, metabolism* Immunohistochemistry / methods Male Mice Mice, Inbred BALB C Neural Pathways / cytology, metabolism Neurons / cytology, metabolism* Neuropeptides / chemistry, immunology, metabolism* Optic Lobe, Nonmammalian / cytology, metabolism* Protein Isoforms / chemistry, immunology, metabolism Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Drosophila Proteins; 0/Epitopes; 0/Neuropeptides; 0/Protein Isoforms; 0/pdf protein, Drosophila |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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