Document Detail


Structural isoforms of the circadian neuropeptide PDF expressed in the optic lobes of the cricket Gryllus bimaculatus: immunocytochemical evidence from specific monoclonal antibodies.
MedLine Citation:
PMID:  16998911     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pigment-dispersing factor (PDF) is an 18-mer peptide that acts as a principal neurotransmitter of the insect circadian clock. Our previous study, utilizing anti-Uca beta-PDH polyclonal antibody (pAb) to immunolabel the optic lobe of the cricket Gryllus bimaculatus, suggested the existence of an alternative PDF-like peptide in the outer cells of the first neuropile, or lamina (La), which were much less immunoreactive than the inner cells of the second neuropile, the medulla (Me). To obtain structural information about such a PDF-like peptide, we prepared 10 anti-Gryllus PDF monoclonal (mAb) and pAb antibodies and analyzed their detailed epitope specificities. The PDFMe and PDFLa inner cells and their axonal projections were clearly immunoreactive to all these antibodies, revealing the widespread immunocytochemical organization of the PDF system in the optic lobe, as seen previously with anti-Uca beta-PDH pAb and anti-Gryllus PDF mAb, the epitope structures of which were also clarified in this study. The lamina outer cells, which we found lacked a target pdf mRNA, displayed specific immunoreactivities, indicating that the cells contain a distinct PDF-like peptide possessing both N- and C-terminal structures. These cells were not immunolabeled by some other monoclonal antibodies, however, implying that the PDFLa outer cells have a PDF isoform peptide devoid of Asn at positions 6 and 16. This isoform was also identified in a varicose arborization in the lamina. These results suggest not only the structure of the peptide, but also the possibility of additional functions of this novel PDF isoform.
Authors:
Takeshi Honda; Ayami Matsushima; Kazunori Sumida; Yoshiro Chuman; Kazuyasu Sakaguchi; Hitoshi Onoue; Ian A Meinertzhagen; Yasuyuki Shimohigashi; Miki Shimohigashi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  499     ISSN:  0021-9967     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-02     Completed Date:  2006-11-24     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  404-21     Citation Subset:  IM    
Affiliation:
Laboratory of Structure-Function Biochemistry, Department of Chemistry, Faculty and Graduate School of Sciences, Kyushu University, Fukuoka 812-8581, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology
Antibody Specificity / immunology
Axons / metabolism,  ultrastructure
Brain / cytology,  metabolism*
Circadian Rhythm / physiology
Drosophila Proteins / chemistry,  immunology,  metabolism
Epitope Mapping / methods
Epitopes / chemistry,  immunology
Gryllidae / anatomy & histology,  metabolism*
Immunohistochemistry / methods
Male
Mice
Mice, Inbred BALB C
Neural Pathways / cytology,  metabolism
Neurons / cytology,  metabolism*
Neuropeptides / chemistry,  immunology,  metabolism*
Optic Lobe, Nonmammalian / cytology,  metabolism*
Protein Isoforms / chemistry,  immunology,  metabolism
Species Specificity
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Drosophila Proteins; 0/Epitopes; 0/Neuropeptides; 0/Protein Isoforms; 0/pdf protein, Drosophila

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