| Structural investigations and identification of the extracellular bacteriolytic endopeptidase L1 from Lysobacter sp. XL1. | |
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MedLine Citation:
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PMID: 15193123 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The N-terminal amino acid sequence (23 amino acid residues) and the amino acid composition of the extracellular bacteriolytic enzyme L1 of 21 kD from the bacterium Lysobacter sp. XL1 have been determined. The enzyme was hydrolyzed by trypsin, the resulting peptides were isolated, and their primary structures were determined. A high extent of homology (92%) of the N-terminal amino acid sequence and the primary structure of isolated peptides of the enzyme L1 (62 amino acid residues or 31% of protein sequence) to the corresponding sites of alpha-lytic proteinases (EC 3.4.21.12) of Lysobacter enzymogenes and Achromobacter lyticus was found. These data allowed identification of the endopeptidase L1 of Lysobacter sp. XL1 as alpha-lytic proteinase EC 3.4.21.12. |
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Authors:
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T A Muranova; L A Krasovskaya; I M Tsfasman; O A Stepnaya; I S Kulaev |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry. Biokhimii͡a Volume: 69 ISSN: 0006-2979 ISO Abbreviation: Biochemistry Mosc. Publication Date: 2004 May |
Date Detail:
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Created Date: 2004-06-14 Completed Date: 2005-04-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376536 Medline TA: Biochemistry (Mosc) Country: United States |
Other Details:
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Languages: eng Pagination: 501-5 Citation Subset: IM |
Affiliation:
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Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, 142290, Moscow Region, Russia. Muranova@fibkh.serpukhov.su |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Achromobacter
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enzymology Amino Acid Sequence Bacteriolysis / physiology* Molecular Sequence Data Peptide Fragments / chemistry Serine Endopeptidases / chemistry* Xanthomonadaceae / enzymology* |
| Chemical | |
Reg. No./Substance:
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0/Peptide Fragments; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.12/myxobacter alpha-lytic proteinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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