| Structural investigation of an exopolysaccharide substituted with a lactyl ether group produced by Raoultella terrigena Ez-555-6 isolated in the Chernobyl exclusion zone. | |
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MedLine Citation:
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PMID: 20417501 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Raoultella terrigena strain Ez-555-6, isolated from a root nodule of Medicago sativa harvested in the Chernobyl exclusion zone, produces a non-referenced high-molecular-mass exopolysaccharide (EPS). The structure of this EPS was determined using a combination approach including monosaccharide composition (GLC-FID, HPAEC-PAD), determination of glycosylation sites (GLC-EIMS) and 1D/2D NMR ((1)H, (13)C) and ESIMS (HR, MS/MS) studies of oligosaccharides obtained from mild acid hydrolysis. The EPS was found to be a charged pentasaccharide with a repeating unit composed of D-galactose, D-glucose, D-mannose and D-glucuronic acid (1:2:1:1). Lactic acid and O-acetyl substituents were localized on galactose and glucose residues, respectively, as presented in the following structure: |
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Authors:
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M Pillon; C Pau-Roblot; V Lequart; S Pilard; B Courtois; J Courtois; N Pawlicki-Jullian |
Publication Detail:
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Type: Journal Article Date: 2010-03-20 |
Journal Detail:
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Title: Carbohydrate research Volume: 345 ISSN: 1873-426X ISO Abbreviation: Carbohydr. Res. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-10 Completed Date: 2010-09-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0043535 Medline TA: Carbohydr Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1163-73 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Laboratoire des Polysaccharides Microbiens et Végétaux, Université de Picardie Jules Verne, IUT d'Amiens, Avenue des Facultés, Amiens, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carbohydrate Sequence Chernobyl Nuclear Accident* Enterobacteriaceae / chemistry* Ethers / chemistry* Glycosides / chemistry Hydrolysis Magnetic Resonance Spectroscopy Molecular Sequence Data Monosaccharides / chemistry Polysaccharides, Bacterial / chemistry*, isolation & purification* Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry |
| Chemical | |
Reg. No./Substance:
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0/Ethers; 0/Glycosides; 0/Monosaccharides; 0/Polysaccharides, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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