| Structural insights into the adaptation of proliferating cell nuclear antigen (PCNA) from Haloferax volcanii to a high-salt environment. | |
| | |
MedLine Citation:
|
PMID: 19770505 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The sliding clamp proliferating cell nuclear antigen (PCNA) plays vital roles in many aspects of DNA replication and repair in eukaryotic cells and in archaea. Realising the full potential of archaea as a model for PCNA function requires a combination of biochemical and genetic approaches. In order to provide a platform for subsequent reverse genetic analysis, PCNA from the halophilic archaeon Haloferax volcanii was subjected to crystallographic analysis. The gene was cloned and expressed in Escherichia coli and the protein was purified by affinity chromatography and crystallized by the vapour-diffusion technique. The structure was determined by molecular replacement and refined at 3.5 A resolution to a final R factor of 23.7% (R(free) = 25%). PCNA from H. volcanii was found to be homotrimeric and to resemble other homotrimeric PCNA clamps but with several differences that appear to be associated with adaptation of the protein to the high intracellular salt concentrations found in H. volcanii cells. |
| | |
Authors:
|
Ekaterina Morgunova; Fiona C Gray; Stuart A Macneill; Rudolf Ladenstein |
Related Documents
:
|
11254985 - Perpendicular planes of ftsz arcs in spheroidal escherichia coli cells. 361715 - Cell division during nutritional upshifts of escherichia coli. 16077105 - Coordinated replication and sequestration of oric and dnaa are required for maintaining... 6339465 - Glycerol and ethylene glycol: members of a new class of repellents of escherichia coli ... 15182695 - The origin of asymmetry: early polarisation of the drosophila germline cyst and oocyte. 15708555 - Oocyte control of metabolic cooperativity between oocytes and companion granulosa cells... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-09-16 |
Journal Detail:
|
Title: Acta crystallographica. Section D, Biological crystallography Volume: 65 ISSN: 1399-0047 ISO Abbreviation: Acta Crystallogr. D Biol. Crystallogr. Publication Date: 2009 Oct |
Date Detail:
|
Created Date: 2009-09-22 Completed Date: 2010-01-26 Revised Date: 2013-03-05 |
Medline Journal Info:
|
Nlm Unique ID: 9305878 Medline TA: Acta Crystallogr D Biol Crystallogr Country: England |
Other Details:
|
Languages: eng Pagination: 1081-8 Citation Subset: IM |
Affiliation:
|
Karolinska Institutet, NOVUM, Centre of Structural Biochemistry, S-14157 Huddinge, Sweden. ekaterina.morgunova@ki.se |
| Data Bank Information | |
Bank Name/Acc. No.:
|
PDB/3HI8; R3HI8SF |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Cloning, Molecular Crystallography, X-Ray* Escherichia coli / genetics Haloferax volcanii / chemistry*, metabolism Models, Molecular Molecular Sequence Data Proliferating Cell Nuclear Antigen / chemistry*, genetics, metabolism Salts / metabolism Sequence Alignment |
| Chemical | |
Reg. No./Substance:
|
0/Proliferating Cell Nuclear Antigen; 0/Salts |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: electronic Ligand Builder and Optimization Workbench (eLBOW): a tool for ligand coordinate and restr...
Next Document: On the combination of molecular replacement and single-wavelength anomalous diffraction phasing for ...