Document Detail

Structural and inflammatory heterogeneity in subcutaneous adipose tissue: relation with liver histopathology in morbid obesity.
MedLine Citation:
PMID:  22245892     Owner:  NLM     Status:  Publisher    
BACKGROUND//AIM: In addition to total body fat, the regional distribution and inflammatory status of enlarged adipose tissue are strongly associated with metabolic co-morbidities of obesity. We recently showed that the severity of histological liver lesions related to obesity increases with the amounts of macrophage accumulation in visceral adipose tissue (VAT), while no association was found with the subcutaneous adipose tissue (SAT). In the abdominal region, SAT is anatomically divided in two layers, i.e. superficial (sSAT) and deep (dSAT) SAT. The aim of the present study was to test the hypothesis that these distinct compartments differentially contribute to hepatic alterations in obesity. METHODS: Biopsies of liver, sSAT, dSAT and VAT were collected in 45 subjects with morbid obesity (Age 43.7 ± 1.6 years; BMI 48.5 ± 1.2 kg/m(2)) during bariatric surgery. Large scale gene expression analysis was performed to identify the pathways that discriminate sSAT from dSAT. Adipose tissue macrophages were quantified by immunohistochemistry using HAM56 antibody in subjects scored for liver histopathology. RESULTS: An inflammatory gene pattern discriminates between sSAT and dSAT. dSAT displayed an intermediate level of macrophage accumulation between sSAT and VAT. The abundance of macrophages in dSAT, but not in sSAT, was significantly increased in patients with non-alcoholic steatohepatitis (NASH) and/or fibroinflammatory hepatic lesions. CONCLUSION: These data show distinct gene signature and macrophage abundance in the two compartments of SAT, with dSAT more closely related to VAT than to sSAT in terms of inflammation and relation with the severity of liver diseases in morbid obesity.
Joan Tordjman; Adeline Divoux; Edi Prifti; Christine Poitou; Veronique Pelloux; Danielle Hugol; Arnaud Basdevant; Jean-Luc Bouillot; Jean-Marc Chevallier; Pierre Bedossa; Michèle Guerre-Millo; Karine Clement
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-12
Journal Detail:
Title:  Journal of hepatology     Volume:  -     ISSN:  0168-8278     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8503886     Medline TA:  J Hepatol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
ICAN Institut Cardiométabolisme et Nutrition, Paris, Pitié-Salpêtrière Hospital, Paris, F-75013 France.; INSERM, U872, Nutriomique, Paris, F-75006 France;Université Pierre et Marie Curie-Paris6, Centre de Recherche des Cordeliers, UMR S 872, Paris, F-75006 France.
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