Document Detail


Structural genes for nitrate-inducible formate dehydrogenase in Escherichia coli K-12.
MedLine Citation:
PMID:  2168848     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Formate oxidation coupled to nitrate reduction constitutes a major anaerobic respiratory pathway in Escherichia coli. This respiratory chain consists of formate dehydrogenase-N, quinone, and nitrate reductase. We have isolated a recombinant DNA clone that likely contains the structural genes, fdnGHI, for the three subunits of formate dehydrogenase-N. The fdnGHI clone produced proteins of 110, 32 and 20 kDa which correspond to the subunit sizes of purified formate dehydrogenase-N. Our analysis indicates that fdnGHI is organized as an operon. We mapped the fdn operon to 32 min on the E. coli genetic map, close to the genes for cryptic nitrate reductase (encoded by the narZ operon). Expression of phi(fdnG-lacZ) operon fusions was induced by anaerobiosis and nitrate. This induction required fnr+ and narL+, two regulatory genes whose products are also required for the anaerobic, nitrate-inducible activation of the nitrate reductase structural gene operon, narGHJI. We conclude that regulation of fdnGHI and narGHJI expression is mediated through common pathways.
Authors:
B L Berg; V Stewart
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  125     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1990-10-18     Completed Date:  1990-10-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  691-702     Citation Subset:  IM    
Affiliation:
Section of Microbiology, Cornell University, Ithaca, New York 14853-8101.
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MeSH Terms
Descriptor/Qualifier:
Aldehyde Oxidoreductases / genetics*
Cloning, Molecular
DNA Transposable Elements
Enzyme Induction
Escherichia coli / enzymology,  genetics*
Escherichia coli Proteins
Formate Dehydrogenases / biosynthesis,  genetics*
Gene Expression Regulation, Bacterial*
Genes, Bacterial*
Genes, Regulator
Models, Genetic
Mutation
Nitrate Reductase
Nitrate Reductases / genetics
Nitrates / pharmacology*
Operon
Oxidation-Reduction
Restriction Mapping
Grant Support
ID/Acronym/Agency:
GM36877/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA Transposable Elements; 0/Escherichia coli Proteins; 0/Nitrates; EC 1.2.-/Aldehyde Oxidoreductases; EC 1.2.1.2/Formate Dehydrogenases; EC 1.7.-/Nitrate Reductases; EC 1.7.99.4/Nitrate Reductase; EC 1.7.99.4/narZ protein, E coli
Comments/Corrections

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