| Structural genes for nitrate-inducible formate dehydrogenase in Escherichia coli K-12. | |
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MedLine Citation:
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PMID: 2168848 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Formate oxidation coupled to nitrate reduction constitutes a major anaerobic respiratory pathway in Escherichia coli. This respiratory chain consists of formate dehydrogenase-N, quinone, and nitrate reductase. We have isolated a recombinant DNA clone that likely contains the structural genes, fdnGHI, for the three subunits of formate dehydrogenase-N. The fdnGHI clone produced proteins of 110, 32 and 20 kDa which correspond to the subunit sizes of purified formate dehydrogenase-N. Our analysis indicates that fdnGHI is organized as an operon. We mapped the fdn operon to 32 min on the E. coli genetic map, close to the genes for cryptic nitrate reductase (encoded by the narZ operon). Expression of phi(fdnG-lacZ) operon fusions was induced by anaerobiosis and nitrate. This induction required fnr+ and narL+, two regulatory genes whose products are also required for the anaerobic, nitrate-inducible activation of the nitrate reductase structural gene operon, narGHJI. We conclude that regulation of fdnGHI and narGHJI expression is mediated through common pathways. |
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Authors:
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B L Berg; V Stewart |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Genetics Volume: 125 ISSN: 0016-6731 ISO Abbreviation: Genetics Publication Date: 1990 Aug |
Date Detail:
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Created Date: 1990-10-18 Completed Date: 1990-10-18 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0374636 Medline TA: Genetics Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 691-702 Citation Subset: IM |
Affiliation:
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Section of Microbiology, Cornell University, Ithaca, New York 14853-8101. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aldehyde Oxidoreductases
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genetics* Cloning, Molecular DNA Transposable Elements Enzyme Induction Escherichia coli / enzymology, genetics* Escherichia coli Proteins Formate Dehydrogenases / biosynthesis, genetics* Gene Expression Regulation, Bacterial* Genes, Bacterial* Genes, Regulator Models, Genetic Mutation Nitrate Reductase Nitrate Reductases / genetics Nitrates / pharmacology* Operon Oxidation-Reduction Restriction Mapping |
| Grant Support | |
ID/Acronym/Agency:
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GM36877/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA Transposable Elements; 0/Escherichia coli Proteins; 0/Nitrates; EC 1.2.-/Aldehyde Oxidoreductases; EC 1.2.1.2/Formate Dehydrogenases; EC 1.7.-/Nitrate Reductases; EC 1.7.99.4/Nitrate Reductase; EC 1.7.99.4/narZ protein, E coli |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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