Document Detail


Structural and functional effects of hydrostatic pressure on centrosomes from vertebrate cells.
MedLine Citation:
PMID:  11276075     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In an attempt to better understand the role of centrioles in vertebrate centrosomes, hydrostatic pressure was applied to isolated centrosomes as a means to disassemble centriole microtubules. Treatments of the centrosomes were monitored by analyzing their protein composition, ultrastructure, their ability to nucleate microtubules from pure tubulin, and their capability to induce parthenogenetic development of Xenopus eggs. Moderate hydrostatic pressure (95 MPa) already affected the organization of centriole microtubules in isolated centrosomes, and also impaired microtubule nucleation. At higher pressure, the protein composition of the peri-centriolar matrix (PCM) was also altered and the capacity to nucleate microtubules severely impaired. Incubation of the treated centrosomes in Xenopus egg extract could restore their capacity to nucleate microtubules after treatment at 95 MPa, but not after higher pressure treatment. However, the centriole structure was in no case restored. It is noteworthy that centrosomes treated with mild pressure did not allow parthenogenetic development after injection into Xenopus eggs, even if they had recovered their capacity to nucleate microtubules. This suggested that, in agreement with previous results, centrosomes in which centriole architecture is impaired, could not direct the biogenesis of new centrioles in Xenopus eggs. Centriole structure could also be affected by applying mild hydrostatic pressure directly to living cells. Comparison of the effect of hydrostatic pressure on cells at the G1/S border or on the corresponding cytoplasts suggests that pro-centrioles are very sensitive to pressure. However, cells can regrow a centriole after pressure-induced disassembly. In that case, centrosomes eventually recover an apparently normal duplication cycle although with some delay.
Authors:
A Rousselet; U Euteneuer; N Bordes; T Ruiz; G Hui Bon Hua; M Bornens
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell motility and the cytoskeleton     Volume:  48     ISSN:  0886-1544     ISO Abbreviation:  Cell Motil. Cytoskeleton     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-03-29     Completed Date:  2001-05-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8605339     Medline TA:  Cell Motil Cytoskeleton     Country:  United States    
Other Details:
Languages:  eng     Pagination:  262-76     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Wiley-Liss, Inc.
Affiliation:
Laboratoire du cycle cellulaire et de la motilité, UMR144-CNRS, Institut Curie, Paris, France. annie.rousselet@curie.fr
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division / physiology
Centrosome / physiology*,  ultrastructure
Fibroblasts / cytology,  physiology
Hela Cells
Humans
Hydrostatic Pressure
Mice
Microtubules / physiology*
Ovum / cytology,  physiology*
Parthenogenesis / physiology
Vertebrates
Xenopus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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