Document Detail


Structural and functional characterization of noncoding repetitive RNAs transcribed in stressed human cells.
MedLine Citation:
PMID:  15788562     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thermal and chemical stresses induce the formation in human cells of novel and transient nuclear structures called nuclear stress bodies (nSBs). These contain heat shock factor 1 (HSF-1) and a specific subset of pre-mRNA processing factors. Nuclear stress bodies are assembled on specific pericentromeric heterochromatic domains containing satellite III (SatIII) DNA. In response to stress, these domains change their epigenetic status from heterochromatin to euchromatin and are transcribed in poly-adenylated RNAs that remain associated with nSBs. In this article, we describe the cloning, sequencing, and functional characterization of these transcripts. They are composed of SatIII repeats and originate from the transcription of multiple sites within the SatIII arrays. Interestingly, the level of SatIII RNAs can be down-regulated both by antisense oligonucleotides and small interfering RNAs (siRNA). Knockdown of SatIII RNA by siRNAs requires the activity of Argonaute 2, a component of the RNA-induced silencing complex. Down-regulation of satellite III RNAs significantly affects the recruitment of RNA processing factors to nSBs without altering the association of HSF-1 with these structures nor the presence of acetylated histones within nSBs. Thus, satellite III RNAs have a major role in the formation of nSBs.
Authors:
Rut Valgardsdottir; Ilaria Chiodi; Manuela Giordano; Fabio Cobianchi; Silvano Riva; Giuseppe Biamonti
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-03-23
Journal Detail:
Title:  Molecular biology of the cell     Volume:  16     ISSN:  1059-1524     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-06-03     Completed Date:  2005-11-21     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2597-604     Citation Subset:  IM    
Affiliation:
Istituto di Genetica Molecolare, Consiglio Nazionale delle Ricerche, 27100 Pavia, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Argonaute Proteins
Cell Fusion
Cell Nucleus / genetics,  metabolism
Cell Nucleus Structures / metabolism*
Coculture Techniques
DNA, Satellite / chemistry,  metabolism
DNA-Binding Proteins
Dose-Response Relationship, Drug
Down-Regulation
Euchromatin / genetics,  metabolism
Eukaryotic Initiation Factor-2
HeLa Cells
Heat-Shock Response
Heterochromatin / genetics,  metabolism
Humans
Mice
NIH 3T3 Cells
Oligonucleotides, Antisense / pharmacology
Peptide Initiation Factors / metabolism
RNA, Small Interfering / pharmacology
RNA, Untranslated / chemistry*,  genetics,  metabolism*
RNA-Induced Silencing Complex
Sequence Analysis, RNA
Stress, Physiological / genetics*,  metabolism
Transcription Factors
Transcription, Genetic*
Chemical
Reg. No./Substance:
0/Argonaute Proteins; 0/DNA, Satellite; 0/DNA-Binding Proteins; 0/EIF2C2 protein, human; 0/Euchromatin; 0/Eukaryotic Initiation Factor-2; 0/Heterochromatin; 0/Oligonucleotides, Antisense; 0/Peptide Initiation Factors; 0/RNA, Small Interfering; 0/RNA, Untranslated; 0/RNA-Induced Silencing Complex; 0/Transcription Factors; 0/heat shock transcription factor
Comments/Corrections

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