Document Detail


Structural and functional aspects of biological freezing techniques.
MedLine Citation:
PMID:  342699     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cooling procedures used to prepare samples for ultrastructural examination at low temperatures often differ markedly from those used to recover optimal function of cells on thawing. The implications of these differences are reviewed. Damage and alteration to the structure and function of the cells may be caused by the high concentrations of cryoprotective agents such as glycerol or dimethyl sulphoxide (DMSO) often added to reduce ice crystal artefacts. Under the rapid cooling conditions commonly employed for structural studies, these additives are not cryoprotective; low rates of cooling are necessary for them to be effective. Rapidly cooled cells that contain intracellular ice are only injured during rewarming so their structure may be as yet unaltered by any damaging effects at low temperatures. Most cells able to recover on thawing are grossly shrunken at low temperatures but since they are potentially functional they are of interest structurally. These cryobiological principles are illustrated with freeze-fracture, freeze substitution and functional assays. The cell types chosen were Chlorella sp. and mammalian tissue culture cells.
Authors:
J Farrant; C A Walter; H Lee; G J Morris; K J Clarke
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of microscopy     Volume:  111     ISSN:  0022-2720     ISO Abbreviation:  J Microsc     Publication Date:  1977 Sep 
Date Detail:
Created Date:  1978-04-26     Completed Date:  1978-04-26     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  17-34     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cricetinae
Cryoprotective Agents / adverse effects
Cytological Techniques*
Dimethyl Sulfoxide / pharmacology
Fibroblasts / ultrastructure
Freeze Fracturing
Freezing
Glycerol / pharmacology
Molecular Weight
Time Factors
Tissue Preservation / methods*
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 56-81-5/Glycerol; 67-68-5/Dimethyl Sulfoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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