| Structural and functional analyses of Saccharomyces cerevisiae wild-type and mutant RNA1 genes. | |
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MedLine Citation:
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PMID: 2674676 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The yeast gene RNA1 has been defined by the thermosensitive rna1-1 lesion. This lesion interferes with the processing and production of all major classes of RNA. Each class of RNA is affected at a distinct and presumably unrelated step. Furthermore, RNA does not appear to exit the nucleus. To investigate how the RNA1 gene product can pleiotropically affect disparate processes, we undertook a structural analysis of wild-type and mutant RNA1 genes. The wild-type gene was found to contain a 407-amino-acid open reading frame that encodes a hydrophilic protein. No clue regarding the function of the RNA1 protein was obtained by searching banks for similarity to other known gene products. Surprisingly, the rna1-1 lesion was found to code for two amino acid differences from wild type. We found that neither single-amino-acid change alone resulted in temperature sensitivity. The carboxy-terminal region of the RNA1 open reading frame contains a highly acidic domain extending from amino acids 334 to 400. We generated genomic deletions that removed C-terminal regions of this protein. Deletion of amino acids 397 to 407 did not appear to affect cell growth. Removal of amino acids 359 to 397, a region containing 24 acidic residues, caused temperature-sensitive growth. This allele, rna1-delta 359-397, defines a second conditional lesion of the RNA1 locus. We found that strains possessing the rna1-delta 359-397 allele did not show thermosensitive defects in pre-rRNA or pre-tRNA processing. Removal of amino acids 330 to 407 resulted in loss of viability. |
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Authors:
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H M Traglia; N S Atkinson; A K Hopper |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular and cellular biology Volume: 9 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 1989 Jul |
Date Detail:
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Created Date: 1989-10-13 Completed Date: 1989-10-13 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2989-99 Citation Subset: IM |
Affiliation:
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Department of Biological Chemistry, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/M27142; M27143 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Base Sequence Blotting, Southern Cloning, Molecular Escherichia coli / genetics Fungal Proteins / genetics*, metabolism GTPase-Activating Proteins* Genes, Fungal* Molecular Sequence Data Mutation* Phenotype Plasmids RNA Processing, Post-Transcriptional RNA, Fungal / genetics*, metabolism Restriction Mapping Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae Proteins Temperature |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 0/GTPase-Activating Proteins; 0/RNA, Fungal; 0/RNA1 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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