Document Detail


Structural evidence that endothelial cell growth factor beta is the precursor of both endothelial cell growth factor alpha and acidic fibroblast growth factor.
MedLine Citation:
PMID:  3532107     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two endothelial cell growth factors (ECGF) have been purified from bovine brain and termed alpha- and beta-ECGF [Burgess, W. H., Mehlman, T., Friesel, R., Johnson, W. V. & Maciag, T. (1985) J. Biol. Chem. 260, 11389-11392]. Amino acid sequence analysis indicates that beta-ECGF represents a 20 amino acid amino-terminal extension of alpha-ECGF and a 14 amino acid amino-terminal extension of acidic fibroblast growth factor. These data indicate that both alpha-ECGF and acidic fibroblast growth factor may be derived from beta-ECGF by posttranslational processing. Analysis of the amino-terminal 14 residues of beta-ECGF by fast-atom-bombardment mass spectrometry established the amino acid sequence of this region and the identity of the blocking group at the amino terminus (acetyl).
Authors:
W H Burgess; T Mehlman; D R Marshak; B A Fraser; T Maciag
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  83     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1986 Oct 
Date Detail:
Created Date:  1986-10-30     Completed Date:  1986-10-30     Revised Date:  2010-09-09    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7216-20     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cattle
Cyanogen Bromide
Endothelial Growth Factors
Fibroblast Growth Factors
Growth Substances* / genetics
Mass Spectrometry
Peptide Fragments / analysis
Protein Precursors
Thermolysin
Trypsin
Grant Support
ID/Acronym/Agency:
AG04807/AG/NIA NIH HHS; HL310765/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Endothelial Growth Factors; 0/Growth Substances; 0/Peptide Fragments; 0/Protein Precursors; 506-68-3/Cyanogen Bromide; 62031-54-3/Fibroblast Growth Factors; EC 3.4.21.4/Trypsin; EC 3.4.24.27/Thermolysin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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