Document Detail


Structural elucidation of metabolites of ginkgolic acid in rat liver microsomes by ultra-performance liquid chromatography/electrospray ionization tandem mass spectrometry and hydrogen/deuterium exchange.
MedLine Citation:
PMID:  19462408     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ginkgolic acids have been shown to possess allergenic as well as genotoxic and cytotoxic properties. The question arises whether the metabolism of ginkgolic acids in the liver could decrease or increase their toxicity. In this study, the in vitro metabolism of ginkgolic acid (15:1, GA), one component of ginkgo acids, was investigated as a model compound in Sprague-Dawley rat liver microsomes. The metabolites were analyzed by ultra-performance liquid chromatography coupled with photodiode array detector/negative-ion electrospray ionization tandem mass spectrometry (UPLC-PDA/ESI-MS/MS) and hydrogen/deuterium (H/D) exchange. The result showed that the benzene ring remained unchanged and the oxidations occurred at the side alkyl chain in rat liver microsomes. At least eight metabolites were found. Among them, six phase I metabolites were tentatively identified. This study might be useful for the investigation of toxicological mechanism of ginkgolic acids.
Authors:
Z H Liu; J Chen; L S Yu; H D Jiang; T W Yao; S Zeng
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  23     ISSN:  1097-0231     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-11     Completed Date:  2009-08-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  1899-906     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2009 John Wiley & Sons, Ltd.
Affiliation:
Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, High Pressure Liquid / methods*
Deuterium Exchange Measurement / methods*
Ginkgo biloba / chemistry
Male
Microsomes, Liver / chemistry*,  metabolism
Rats
Rats, Sprague-Dawley
Salicylic Acids / chemistry*,  metabolism
Spectrometry, Mass, Electrospray Ionization / methods*
Tandem Mass Spectrometry
Chemical
Reg. No./Substance:
0/Salicylic Acids; 22910-60-7/ginkgolic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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