Document Detail

Structural difference between the two forms of guinea-pig beta 2-microglobulin and their occurrence in inbred guinea-pig strains.
MedLine Citation:
PMID:  3934525     Owner:  NLM     Status:  MEDLINE    
The structural difference between two forms (basic and acidic) of guinea-pig beta 2-microglobulin (beta 2m) has been established. Both forms are present in urine from inbred guinea-pig strains. The beta 2m forms were each digested with carboxypeptidase Y and carboxypeptidase A contaminated with carboxypeptidase B. Released amino acids were separated from remaining protein, dansylated and analysed by 2-dimensional TLC on polyamide layer sheets. From the results it was concluded that the basic beta 2m form has lysine and the acidic beta 2m form has asparagine as their respective C-terminal amino acids. The acidic form is also 1 amino acid (lysine) shorter than the basic form, which is supported by electrophoretic studies on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The presence of the 2 forms of beta 2m in urine from inbred guinea-pig strains 2 and 13, shown by gel filtration and ion exchange chromatography, makes it unlikely that the 2 forms are a result of genetic polymorphism.
R Cigén
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular immunology     Volume:  22     ISSN:  0161-5890     ISO Abbreviation:  Mol. Immunol.     Publication Date:  1985 Sep 
Date Detail:
Created Date:  1986-01-21     Completed Date:  1986-01-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7905289     Medline TA:  Mol Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1039-43     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Asparagine / analysis
Carboxypeptidases / metabolism
Chromatography, Ion Exchange
Electrophoresis, Polyacrylamide Gel
Guinea Pigs
Lysine / analysis
Peptide Chain Termination, Translational
beta 2-Microglobulin* / urine
Reg. No./Substance:
0/beta 2-Microglobulin; 56-87-1/Lysine; 7006-34-0/Asparagine; EC 3.4.-/Carboxypeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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