| Structural determination of the polar glycoglycerolipids from thermophilic bacteria Meiothermus taiwanensis. | |
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MedLine Citation:
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PMID: 15560795 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The polar glycolipids were isolated from the thermophilic bacteria Meiothermus taiwanensis ATCC BAA-400 by ethanol extraction and purified by Sephadex LH-20 and silica gel column chromatography. The fatty acid composition of O-acyl groups in the glycolipids was obtained by gas chromatography mass spectroscopy analysis on their methyl esters derived from methanolysis and was made mainly of C(15:0) (34.0%) and C(17:0) (42.3%) fatty acids, with the majority as branched fatty acids (over 80%). Removal of O-acyl groups under mild basic conditions provided two glycolipids, which differ only in N-acyl substitution on a hexosamine. Electrospray mass spectroscopy analysis revealed that one has a C(17:0) N-acyl group and the other hydroxy C(17:0) in a ratio of about 1 : 3.5. Furthermore, complete de-lipidation with strong base followed by selective N-acetylation resulted in a homogeneous tetraglycosyl glycerol. The linkages and configurations of the carbohydrate moiety were then elucidated by MS and various NMR analyses. Thus, the major glycolipid from M. taiwanensis ATCC BAA-400 was determined with the following structure: alpha-Galp(1-6)-beta-Galp(1-6)-beta-GalNAcyl(1,2)-alpha-Glc(1,1)-Gro diester, where N-acyl is C(17:0) or hydroxy C(17:0) fatty acid and the glycerol esters were mainly iso- and anteisobranched C(15:0) and C(17:0). |
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Authors:
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Feng-Ling Yang; Chun-Ping Lu; Chien-Sheng Chen; Mao-Yen Chen; Hung-Liang Hsiao; Yeu Su; San-San Tsay; Wei Zou; Shih-Hsiung Wu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of biochemistry / FEBS Volume: 271 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 2004 Nov |
Date Detail:
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Created Date: 2004-11-24 Completed Date: 2005-03-03 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: Germany |
Other Details:
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Languages: eng Pagination: 4545-51 Citation Subset: IM |
Affiliation:
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Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acylation Carbohydrate Conformation Carbohydrate Sequence Deinococcus / chemistry*, genetics Gas Chromatography-Mass Spectrometry Glycerides / chemistry* Glycolipids / chemistry* Methyl Ethers / analysis Nuclear Magnetic Resonance, Biomolecular Spectrometry, Mass, Electrospray Ionization |
| Chemical | |
Reg. No./Substance:
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0/Glycerides; 0/Glycolipids; 0/Methyl Ethers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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