Document Detail

Structural conservation in the major facilitator superfamily as revealed by comparative modeling.
MedLine Citation:
PMID:  15215526     Owner:  NLM     Status:  MEDLINE    
The structures of membrane transporters are still mostly unsolved. Only recently, the first two high-resolution structures of transporters of the major facilitator superfamily (MFS) were published. Despite the low sequence similarity of the two proteins involved, lactose permease and glycerol-3-phosphate transporter, the reported structures are highly similar. This leads to the hypothesis that all members of the MFS share a similar structure, regardless of their low sequence identity. To test this hypothesis, we generated models of two other members of the MFS, the Tn10-encoded metal-tetracycline/H(+) antiporter (TetAB) and the rat vesicular monoamine transporter (rVMAT2). The models are based on the two MFS structures and on experimental data. The models for both proteins are in good agreement with the data available and support the notion of a shared fold for all MFS proteins.
Eyal Vardy; Isaiah T Arkin; Kay E Gottschalk; H Ronald Kaback; Shimon Schuldiner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Protein science : a publication of the Protein Society     Volume:  13     ISSN:  0961-8368     ISO Abbreviation:  Protein Sci.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-06-24     Completed Date:  2005-01-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9211750     Medline TA:  Protein Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1832-40     Citation Subset:  IM    
Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, 91904 Israel.
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MeSH Terms
Antiporters / chemistry*
Bacterial Proteins / chemistry*
Computer Simulation*
Membrane Glycoproteins / chemistry*
Membrane Transport Proteins / chemistry*
Structural Homology, Protein*
Vesicular Biogenic Amine Transport Proteins
Vesicular Monoamine Transport Proteins
Grant Support
Reg. No./Substance:
0/Antiporters; 0/Bacterial Proteins; 0/Membrane Glycoproteins; 0/Membrane Transport Proteins; 0/Vesicular Biogenic Amine Transport Proteins; 0/Vesicular Monoamine Transport Proteins; 0/tetA protein, Bacteria

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