Document Detail

Structural comparison of monoclonal antibody immunopurified pulmonary and hepatic cytochrome P-450 from 3-methylcholanthrene-treated rats.
MedLine Citation:
PMID:  3778507     Owner:  NLM     Status:  MEDLINE    
A pulmonary cytochrome P-450 was purified from lung microsomes of 3-methylcholanthrene (MC)-treated rats by immunoaffinity chromatography using a monoclonal antibody to MC-induced rat liver cytochrome P-450. The isolated pulmonary cytochrome P-450 was MC-inducible and had an apparent molecular weight of 57 kD on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular weight, as well as the NH2-terminal sequence of the first nine amino acids of the pulmonary cytochrome P-450, was identical to that of an epitopically related MC-induced rat liver cytochrome P-450. In addition, partial proteolysis of both cytochromes P-450 yielded indistinguishable peptide patterns on SDS-Page. Treatment of rats with MC, therefore, induces a pulmonary cytochrome P-450 which is structurally identical to the MC-induced hepatic enzyme by several criteria.
R C Robinson; K C Cheng; S S Park; H V Gelboin; F K Friedman
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biochemical pharmacology     Volume:  35     ISSN:  0006-2952     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  1986 Nov 
Date Detail:
Created Date:  1986-11-28     Completed Date:  1986-11-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  3827-30     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Antibodies, Monoclonal / diagnostic use
Cytochrome P-450 Enzyme System / analysis*
Lung / analysis*,  drug effects
Methylcholanthrene / pharmacology*
Microsomes, Liver / analysis*,  drug effects
Rats, Inbred Strains
Reg. No./Substance:
0/Antibodies, Monoclonal; 56-49-5/Methylcholanthrene; 9035-51-2/Cytochrome P-450 Enzyme System

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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