Document Detail


Structural chromosomal aberrations, aneuploidy, and mosaicism in early cleavage mouse embryos derived from spermatozoa exposed to γ-rays.
MedLine Citation:
PMID:  21087169     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Purpose: To quantitatively and qualitatively investigate the changes in chromosomal aberrations during early cleavage in mouse embryos derived from γ-irradiated spermatozoa. Materials and methods: Mature males were exposed to 2 Gy or 4 Gy of (137)Cs γ-rays, and their spermatozoa were used to produce embryos via in vitro fertilisation (IVF). The metaphase chromosomes were prepared from one-cell, two-cell, and four-cell embryos. In the chromosome preparations from two-cell and four-cell embryos, the separation of the sister blastomeres was precluded by treatment of the embryos with concanavalin A. The incidence of embryos with structural chromosomal aberrations, aneuploidy, or mosaicism was estimated. The fates of the different types of γ-ray-induced structural chromosomal aberrations were also investigated in those embryos. Results: The exposure of spermatozoa to 2 Gy or 4 Gy γ-rays caused structural chromosomal aberrations in 25.9% and 35.7% of the resultant one-cell embryos, respectively. At two-cell embryonic stage, the incidence of structural chromosomal aberrations was 17.4% in the 2 Gy group and 27.1% in the 4 Gy group. At the four-cell embryonic stage, although the incidence of control embryos with structural chromosomal aberrations was considerably high, the net incidence of embryos with radiation-induced structural chromosomal aberrations was similar to that at the one-cell stage. The incidence of aneuploidy was high in two-cell and four-cell embryos after both doses of γ-rays. The incidence of mosaicism increased significantly in dose- and embryonic-stage-dependent manners. Anaphase lag, and the degeneration and non-disjunction of the aberrant chromosomes were frequently observed in aneuploid and mosaic embryos. Conclusions: Mouse sperm DNA is highly vulnerable to γ-rays. The structural chromosomal aberrations of sperm origin are unstable in their behaviour and structure during cleavage, and therefore cause secondary aneuploidy and mosaicism in the early cleavage embryos.
Authors:
Hiroyuki Tateno; Hirokazu Kusakabe; Yujiroh Kamiguchi
Related Documents :
21486369 - Predicting the probability of outbreeding depression.
20718579 - Rapid aneuploidy testing versus traditional karyotyping in amniocentesis for certain re...
8465859 - Unusual lymphangioma observed prenatally in a 45,x fetus.
16725589 - A cytogenetical study of prenatal loss in the mare.
21174539 - Runx1 translocations and fusion genes in malignant hemopathies.
6460779 - Age of ascertainment: an index of relative severity of cytogenetic conditions.
3148489 - Genes controlling malathion resistance in a laboratory-selected population of drosophil...
17003069 - Progranulin gene mutations associated with frontotemporal dementia and progressive non-...
7603439 - Chromosome polymorphisms close to the cm-ade1 locus of candida maltosa.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-11-19
Journal Detail:
Title:  International journal of radiation biology     Volume:  -     ISSN:  1362-3095     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Biological Sciences, Asahikawa Medical University, Asahikawa, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Protection by methylproamine of irradiated human keratinocytes correlates with reduction of DNA dama...
Next Document:  The influence of irradiation on the potential chondroprotective effect of aqueous extract of propoli...