Document Detail


Structural and chemical complementarity between antibodies and the crystal surfaces they recognize.
MedLine Citation:
PMID:  10024024     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The sequences of the variable regions of three monoclonal antibodies with different specificities to cholesterol monohydrate and 1,4-dinitrobenzene crystals were determined. The structures of their binding sites were then modeled, based on homology to other antibodies of known structure. Two of these antibodies were previously shown to specifically recognize each one well-defined face of one of the crystals, out of a number of crystal faces of closely related structure. The binding site of the antibody which recognizes the stepped (301) face of the cholesterol crystal is predicted to assume the shape of a step with one hydrophobic and one hydrophilic side, complementary to the corresponding crystal surface. Within the step, the hydroxyl groups of five tyrosines are located such that they can interact with the hydroxyl and water molecules on the cholesterol crystal face, while hydrophobic contacts are made between the cholesterol backbone and hydrophobic amino acid sidechains. In contrast, the modeled binding site of the antibody which recognizes the flat (101) face of 1,4-dinitrobenzene crystals is remarkably flat. It is lined by aromatic and polar residues, that can make favorable contacts with the aromatic ring and nitro groups of the dinitrobenzene molecules, respectively.
Authors:
N Kessler; D Perl-Treves; L Addadi; M Eisenstein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proteins     Volume:  34     ISSN:  0887-3585     ISO Abbreviation:  Proteins     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-04-23     Completed Date:  1999-04-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8700181     Medline TA:  Proteins     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  383-94     Citation Subset:  IM    
Affiliation:
Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / chemistry*
Antigens / chemistry*
Binding Sites
Cholesterol / chemistry
Cloning, Molecular
Computer Simulation
Crystallization
Dinitrobenzenes / chemistry
Immunoglobulin Variable Region / chemistry,  genetics
Models, Molecular
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens; 0/Dinitrobenzenes; 0/Immunoglobulin Variable Region; 100-25-4/1,4-dinitrobenzene; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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