Document Detail

Structural characterization of the serotype O:5 O-polysaccharide antigen of the lipopolysaccharide of Escherichia coli O:5.
MedLine Citation:
PMID:  9404639     Owner:  NLM     Status:  MEDLINE    
The structure of the antigenic O-polysaccharide component of the smooth lipopolysaccharide produced by Escherichia coli serotype O:5 was investigated by composition, methylation, and periodate oxidation methods, and by 1D and 2D nuclear magnetic resonance spectroscopy. The antigenic O-chain was determined to be a high molecular weight polysaccharide composed of repeating tetrasaccharide units containing 2-acetamido-2-deoxy-D-galactose, D-galactose, D-ribose, and 3-acetamido-3,6-dideoxy-D-glucose and having the structure -[-4)-beta-D-Galp-(1-3)-alpha-D-GalpNAc-(1-4)-beta-D-Quinp3NAc-(1- 3)-beta-D-Ribf-(1-]-.
L L MacLean; M B Perry
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemistry and cell biology = Biochimie et biologie cellulaire     Volume:  75     ISSN:  0829-8211     ISO Abbreviation:  Biochem. Cell Biol.     Publication Date:  1997  
Date Detail:
Created Date:  1998-01-15     Completed Date:  1998-01-15     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  8606068     Medline TA:  Biochem Cell Biol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  199-205     Citation Subset:  IM    
Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada.
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MeSH Terms
Carbohydrate Conformation
Carbohydrate Sequence
Chromatography, Gas
Escherichia coli / chemistry*,  classification*,  immunology
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Molecular Structure
O Antigens / chemistry*,  isolation & purification
Polymers / chemistry,  isolation & purification
Reg. No./Substance:
0/O Antigens; 0/Polymers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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