Document Detail


Structural characterization of a eukaryotic chaperone-the ribosome-associated complex.
MedLine Citation:
PMID:  23202586     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Ribosome-associated chaperones act in early folding events during protein synthesis. Structural information is available for prokaryotic chaperones (such as trigger factor), but structural understanding of these processes in eukaryotes lags far behind. Here we present structural analyses of the eukaryotic ribosome-associated complex (RAC) from Saccharomyces cerevisiae and Chaetomium thermophilum, consisting of heat-shock protein 70 (Hsp70) Ssz1 and the Hsp40 Zuo1. RAC is an elongated complex that crouches over the ribosomal tunnel exit and seems to be stabilized in a distinct conformation by expansion segment ES27. A unique α-helical domain in Zuo1 mediates ribosome interaction of RAC near the ribosomal proteins L22e and L31e and ribosomal RNA helix H59. The crystal structure of the Ssz1 ATPase domain bound to ATP-Mg(2+) explains its catalytic inactivity and suggests that Ssz1 may act before the RAC-associated chaperone Ssb. Our study offers insights into the interplay between RAC, the ER membrane-integrated Hsp40-type protein ERj1 and the signal-recognition particle.
Authors:
Christoph Leidig; Gert Bange; Jürgen Kopp; Stefan Amlacher; Ajay Aravind; Stephan Wickles; Gregor Witte; Ed Hurt; Roland Beckmann; Irmgard Sinning
Related Documents :
24100326 - Structure of the sgt2 dimerization domain complexed with the get5 ubl domain involved i...
24307036 - Assembly of the chlorophyll-protein complexes.
24430806 - Evidence from inhibitor studies that the endophyte synthesises nitrogenase in the root ...
24072706 - Interaction of nascent chains with the ribosomal tunnel proteins rpl4, rpl17, and rpl39...
22105356 - In vitro uncoating of hiv-1 cores.
16193276 - Glucose-1-phosphatase (agpe) from enterobacter cloacae displays enhanced phytase activity.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-02
Journal Detail:
Title:  Nature structural & molecular biology     Volume:  -     ISSN:  1545-9985     ISO Abbreviation:  Nat. Struct. Mol. Biol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101186374     Medline TA:  Nat Struct Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1] Gene Center and Center of Integrated Protein Science Munich (CiPS-M), Department of Chemistry and Biochemistry, University of Munich, Munich, Germany. [2].
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The ATPase domain of ISWI is an autonomous nucleosome remodeling machine.
Next Document:  Structure of measles virus hemagglutinin bound to its epithelial receptor nectin-4.