Document Detail


Structural characterization of the acid-degraded secondary cell wall polymer of Geobacillus stearothermophilus PV72/p2.
MedLine Citation:
PMID:  18420185     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The secondary cell wall polymer (SCWP) from Geobacillus stearothermophilus PV72/p2, which is involved in the anchoring of the surface-layer protein to the bacterial cell wall layer, is composed of 2-amino-2-deoxy- and 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-mannose, and 2-acetamido-2-deoxy-D-mannuronic acid. The primary structure of the acid-degraded polysaccharide--liberated by HF-treatment from the cell wall--was determined by high-field NMR spectroscopy and mass spectrometry using N-acetylated and hydrolyzed polysaccharide derivatives as well as Smith-degradation. The polysaccharide was shown to consist of a tetrasaccharide repeating unit containing a pyruvic acid acetal at a side-chain 2-acetamido-2-deoxy-alpha-D-mannopyranosyl residue. Substoichiometric substitutions of the repeating unit were observed concerning the degree of N-acetylation of glucosamine residues and the presence of side-chain linked 2-acetamido-2-deoxy-beta-D-glucopyranosyl units: [Formula: see text].
Authors:
Bent O Petersen; Margit Sára; Christoph Mader; Harald F Mayer; Uwe B Sleytr; Martin Pabst; Michael Puchberger; Eberhard Krause; Andreas Hofinger; Jens Ø Duus; Paul Kosma
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Publication Detail:
Type:  Journal Article     Date:  2008-03-30
Journal Detail:
Title:  Carbohydrate research     Volume:  343     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-12     Completed Date:  2008-07-29     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1346-58     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Carlsberg Laboratory, Valby, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Carbohydrate Conformation
Carbohydrate Sequence
Cell Wall / chemistry*,  metabolism
Geobacillus stearothermophilus / chemistry*,  metabolism
Hydrofluoric Acid
Hydrolysis
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Polysaccharides, Bacterial / chemistry*,  metabolism
Spectrometry, Mass, Electrospray Ionization
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Chemical
Reg. No./Substance:
0/Polysaccharides, Bacterial; 7664-39-3/Hydrofluoric Acid
Comments/Corrections
Erratum In:
Carbohydr Res. 2013 May 24;373:52

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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