Document Detail

Structural characteristics of a novel antifreeze protein from the longhorn beetle Rhagium inquisitor.
MedLine Citation:
PMID:  21078390     Owner:  NLM     Status:  In-Data-Review    
Antifreeze proteins (AFPs) are characterized by their capacity to inhibit the growth of ice and are produced by a variety of polar fish, terrestrial arthropods and other organisms inhabiting cold environments. This capacity reflects their role as stabilizers of supercooled body fluids. The longhorn beetle Rhagium inquisitor is known to express AFPs in its body fluids. In this work we report on the primary structure and structural characteristics of a 12.8 kDa AFP from this beetle (RiAFP). It has a high capacity to evoke antifreeze activity as compared to other known insect AFPs and it is structurally unique in several aspects. In contrast to the high content of disulfide bond-formation observed in other coleopteran AFPs, RiAFP contains only a single such bond. Six internal repeat segments of a thirteen residue repeat pattern is irregularly spaced apart throughout its sequence. The central part of these repeat segments is preserved as TxTxTxT, which is effectively an expansion of the TxT ice-binding motif found in the AFPs of several known insect AFPs.
E Kristiansen; H Ramløv; P Højrup; S A Pedersen; L Hagen; K E Zachariassen
Publication Detail:
Type:  Journal Article     Date:  2010-11-13
Journal Detail:
Title:  Insect biochemistry and molecular biology     Volume:  41     ISSN:  1879-0240     ISO Abbreviation:  Insect Biochem. Mol. Biol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9207282     Medline TA:  Insect Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  109-17     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Department of Biology, Norwegian University of Science and Technology (NTNU), Trondheim, Norway; Department of Science, Systems and Models, Roskilde University, Roskilde, Denmark.
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