| Structural changes induced by a lytic bacteriophage make ciprofloxacin effective against older biofilm of Klebsiella pneumoniae. | |
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MedLine Citation:
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PMID: 20711894 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bacteria have evolved multiple mechanisms, such as biofilm formation, to thwart antibiotic action. Yet antibiotics remain the drug of choice against clinical infections. It has been documented that young biofilm of Klebsiella pneumoniae could be eradicated significantly by ciprofloxacin treatment alone. Since age of biofilm is a decisive factor in determining the outcome of antibiotic treatment, in the present study biofilm of K. pneumoniae, grown for extended periods was treated with ciprofloxacin and/or depolymerase producing lytic bacteriophage (KPO1K2). The reduction in bacterial numbers of older biofilm was greater after application of the two agents in combination as ciprofloxacin alone could not reduce bacterial biomass significantly in older biofilms (P > 0.05). Confocal microscopy suggested the induction of structural changes in the biofilm matrix and a decrease in micro-colony size after KPO1K2 treatment. The role of phage associated depolymerase was emphasized by the insignificant eradication of biofilm by a non-depolymerase producing bacteriophage that, however, eradicated the biofilm when applied concomitantly with purified depolymerase. These findings demonstrate that a lytic bacteriophage alone can eradicate older biofilms significantly and its action is primarily depolymerase mediated. However, application of phage and antibiotic in combination resulted in slightly increased biofilm eradication confirming the speculation that antibiotic efficacy can be augmented by bacteriophage. |
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Authors:
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Vivek Verma; Kusum Harjai; Sanjay Chhibber |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biofouling Volume: 26 ISSN: 1029-2454 ISO Abbreviation: Biofouling Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-16 Completed Date: 2010-12-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9200331 Medline TA: Biofouling Country: England |
Other Details:
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Languages: eng Pagination: 729-37 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Basic Medical Sciences (BMS) Block, Panjab University, Chandigarh, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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pharmacology* Bacteriophages / physiology* Biofilms / drug effects*, growth & development Ciprofloxacin / pharmacology* Humans Klebsiella pneumoniae / drug effects*, ultrastructure, virology* Microbial Sensitivity Tests Microscopy, Confocal |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 85721-33-1/Ciprofloxacin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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