Document Detail

Structural and biomechanical characterizations of porcine myocardial extracellular matrix.
MedLine Citation:
PMID:  22584822     Owner:  NLM     Status:  MEDLINE    
Extracellular matrix (ECM) of myocardium plays an important role to maintain a multilayered helical architecture of cardiomyocytes. In this study, we have characterized the structural and biomechanical properties of porcine myocardial ECM. Fresh myocardium were decellularized in a rotating bioreactor using 0.1 % sodium dodecyl sulfate solution. Masson's trichrome staining and SEM demonstrated the removal of cells and preservation of the interconnected 3D cardiomyocyte lacunae. Movat's pentachrome staining showed the preservation of cardiac elastin ultrastructure and vascular elastin distribution/alignment. DNA assay result confirmed a 98.59 % reduction in DNA content; the acellular myocardial scaffolds were found completely lack of staining for the porcine α-Gal antigen; and the accelerating enzymatic degradation assessment showed a constant degradation rate. Tensile and shear properties of the acellular myocardial scaffolds were also evaluated. Our observations showed that the acellular myocardial ECM possessed important traits of biodegradable scaffolds, indicating the potentials in cardiac regeneration and whole heart tissue engineering.
Bo Wang; Mary E Tedder; Clara E Perez; Guangjun Wang; Amy L de Jongh Curry; Filip To; Steven H Elder; Lakiesha N Williams; Dan T Simionescu; Jun Liao
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2012-05-15
Journal Detail:
Title:  Journal of materials science. Materials in medicine     Volume:  23     ISSN:  1573-4838     ISO Abbreviation:  J Mater Sci Mater Med     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-20     Completed Date:  2012-12-12     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  9013087     Medline TA:  J Mater Sci Mater Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1835-47     Citation Subset:  IM    
Department of Agricultural and Biological Engineering, Computational Manufacturing and Design, CAVS, Mississippi State University, Starkville, MS 39762, USA.
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MeSH Terms
Cell-Free System / chemistry*,  ultrastructure*
Extracellular Matrix / chemistry*,  ultrastructure*
Materials Testing
Myocardium / chemistry*,  ultrastructure*
Shear Strength
Tensile Strength
Tissue Engineering / methods
Tissue Scaffolds*
Grant Support
HL097321/HL/NHLBI NIH HHS; R01 HL093399/HL/NHLBI NIH HHS; R03 TW008941/TW/FIC NIH HHS; R15 HL097321/HL/NHLBI NIH HHS; R1CRH10429-01-00//PHS HHS

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