| Structural basis for conservation in the CYP51 family. | |
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MedLine Citation:
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PMID: 20547249 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sterol 14α-demethylases (14DM) comprise the CYP51 cytochrome P450 genome family. The 14DM reaction is essential for the biosynthesis of sterols which are necessary for production of cellular membranes. This is the most widely distributed P450, being present in all biological kingdoms. From one kingdom to another the primary amino acid sequence identity usually ranges between 30 and 20%. In this minireview we describe the conservation of specific amino acids and the various CYP51 orthologs and indicate the roles that they may play in the structure/function of this monooxygenase. The prediction of the roles of different amino acids in 14DM is based on high resolution tertiary structures of these enzymes which set the stage for detailed understanding of the 14α-demethylase reaction and its selective, phyla-specific inhibition which is crucial for the design of potent inhibitors for treatment of infection by pathogenic microbes. |
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Authors:
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Galina I Lepesheva; Michael R Waterman |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Review Date: 2010-06-11 |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1814 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-11-26 Completed Date: 2011-03-21 Revised Date: 2013-04-19 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 88-93 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146, USA. galina.i.lepesheva@vanderbilt.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Conserved Sequence / genetics Eukaryotic Cells / enzymology* Humans Molecular Sequence Data Protein Structure, Secondary* Protein Structure, Tertiary* Sequence Homology, Amino Acid Sterol 14-Demethylase / chemistry*, genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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GM067871/GM/NIGMS NIH HHS; R01 GM067871/GM/NIGMS NIH HHS; R01 GM067871-07/GM/NIGMS NIH HHS; R01 GM067871-08/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 1.14.13.70/Sterol 14-Demethylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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