Document Detail

Structural aspects of mammalian DNA replication: topoisomerase II.
MedLine Citation:
PMID:  2819726     Owner:  NLM     Status:  MEDLINE    
The dynamic biology of mammalian DNA requires structural complexity. Previous studies on the structure and function of mammalian DNA have suggested that mammalian cell nuclei contain DNA organized into loop-domains by a nuclear structural subcomponent termed the nuclear matrix. The loop-domains, functioning as replicons during DNA synthesis, appear to be replicated by biosynthetic complexes located at fixed sites within the nuclear matrix milieu. Recently, we have found the mammalian DNA topoisomerase II enzyme to be associated with newly replicated DNA, and we speculate that the enzyme may be strategically positioned to untangle topologically intertwined daughter loop-domains before mitotic segregation. Further studies demonstrating growth-related elevations of DNA topoisomerase II levels in rat prostatic adenocarcinoma tissues support a role for the enzyme in cellular proliferation in vivo. The possible participation of DNA topoisomerase II in mammalian DNA replication and the proliferation-dependent appearance of the enzyme in neoplastic tissues may have important implications for therapeutic strategies directed at DNA topoisomerase II.
W G Nelson; D S Coffey
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  NCI monographs : a publication of the National Cancer Institute     Volume:  -     ISSN:  0893-2751     ISO Abbreviation:  NCI Monogr     Publication Date:  1987  
Date Detail:
Created Date:  1987-10-22     Completed Date:  1987-10-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8610384     Medline TA:  NCI Monogr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  23-9     Citation Subset:  IM    
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MeSH Terms
Cell Nucleus / enzymology*
DNA Replication*
DNA Topoisomerases, Type II / metabolism*
Immunologic Techniques
Models, Genetic
Prostatic Neoplasms / enzymology
Grant Support
Reg. No./Substance:
EC Topoisomerases, Type II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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