Document Detail


Structural analysis of human chromosomes by atomic force and light microscopy in relation to the distribution of topoisomerase IIα.
MedLine Citation:
PMID:  21471659     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The relationship between the higher-order structure of human metaphase chromosomes and the distribution of topoisomerase IIα was analyzed by a comparison of atomic force microscope (AFM) and fluorescence microscope images of the same chromosome. AFM imaging of chromosomes in liquid revealed the presence of alternating ridges and grooves on the surfaces of the sister chromatids. In contrast, the fluorescence image of the chromosomes stained with the anti-topoisomerase IIα antibody showed that the fluorescence intensity of topoisomerase IIα was not uniform and that there were alternating strong and weak spots along the chromosome axes. A comparison of the AFM image with a fluorescence microscope image of the same chromosome further demonstrated that ridges and grooves corresponded to strong and weak fluorescence intensities of topoisomerase IIα, respectively. These findings suggest that the distribution of topoisomerase IIα has a close connection with the higher-order structure of human metaphase chromosomes.
Authors:
Osamu Hoshi; Tatsuo Ushiki
Related Documents :
20041939 - Infantile spasms and pigmentary mosaicism.
21240689 - Mapping of sequence-specific markers and loci controlling preharvest sprouting and alph...
16240299 - Automatic analysis of multiplex ligation-dependent probe amplification products (exempl...
21447089 - Identification of the stat5b-rarα fusion transcript in an acute promyelocytic leukemia...
12813759 - Discordant prenatal diagnosis of trisomy 21 due to mosaic structural rearrangements of ...
21217839 - Effect of occupational exposure to cytostatics and nucleotide excision repair polymorph...
12502269 - Fluorescence in situ hybridization mapping of avena sativa l. cv. sunii and its monosom...
3259339 - Gene encoding human p250 t-cell activation antigen maps to human chromosome 11.
11298979 - The contribution of epistasis to species differences in annual sunflowers.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of histology and cytology     Volume:  72     ISSN:  1349-1717     ISO Abbreviation:  Arch. Histol. Cytol.     Publication Date:  2009  
Date Detail:
Created Date:  2011-04-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806082     Medline TA:  Arch Histol Cytol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  245-9     Citation Subset:  IM    
Affiliation:
Division of Microscopic Anatomy and Bio-imaging, Niigata University Graduate School of Medical and Dental Science.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Wide range scanning probe microscopy for probing mechanical effects on cellular function.
Next Document:  The measurement of biomechanical properties of porcine articular cartilage using atomic force micros...