Document Detail

Structural analysis of human chromosomes by atomic force and light microscopy in relation to the distribution of topoisomerase IIα.
MedLine Citation:
PMID:  21471659     Owner:  NLM     Status:  In-Data-Review    
The relationship between the higher-order structure of human metaphase chromosomes and the distribution of topoisomerase IIα was analyzed by a comparison of atomic force microscope (AFM) and fluorescence microscope images of the same chromosome. AFM imaging of chromosomes in liquid revealed the presence of alternating ridges and grooves on the surfaces of the sister chromatids. In contrast, the fluorescence image of the chromosomes stained with the anti-topoisomerase IIα antibody showed that the fluorescence intensity of topoisomerase IIα was not uniform and that there were alternating strong and weak spots along the chromosome axes. A comparison of the AFM image with a fluorescence microscope image of the same chromosome further demonstrated that ridges and grooves corresponded to strong and weak fluorescence intensities of topoisomerase IIα, respectively. These findings suggest that the distribution of topoisomerase IIα has a close connection with the higher-order structure of human metaphase chromosomes.
Osamu Hoshi; Tatsuo Ushiki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of histology and cytology     Volume:  72     ISSN:  1349-1717     ISO Abbreviation:  Arch. Histol. Cytol.     Publication Date:  2009  
Date Detail:
Created Date:  2011-04-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806082     Medline TA:  Arch Histol Cytol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  245-9     Citation Subset:  IM    
Division of Microscopic Anatomy and Bio-imaging, Niigata University Graduate School of Medical and Dental Science.
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