| Structural analysis of glycosphingolipid analogues obtained by the saccharide primer method using CE-ESI-MS. | |
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MedLine Citation:
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PMID: 19813238 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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A glycosphingolipid analogue (12-azidododecyl beta-lactoside) as a saccharide primer has been shown to be useful for the synthesis of oligosaccharide libraries by mammalian cells. In the present study, CE-ESI-MS was employed to elucidate the structure of glycosphingolipid analogues derived from 12-azidododecyl beta-lactoside (Lac-C12N3) by mammalian cells. MDCK cells and COLO201 cells were cultured with Lac-C12N3, and the glycosylated products secreted into the medium were collected and separated into acidic and neutral products by column chromatography. The acidic products could be directly analyzed by CE-ESI-MS, while the neutral products were converted to anionic derivatives via a reaction with propiolic acid. With this method, it was possible to analyze both acidic and neutral products glycosylated by MDCK cells and COLO201 cells at high sensitivity. |
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Authors:
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Xingyu Zhu; Kenichi Hatanaka; Tatsuya Yamagata; Toshinori Sato |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Electrophoresis Volume: 30 ISSN: 1522-2683 ISO Abbreviation: Electrophoresis Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-11-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: Germany |
Other Details:
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Languages: eng Pagination: 3519-26 Citation Subset: IM |
Affiliation:
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Department of Biosciences and Informatics, Keio University, Yokohama, Japan. |
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