| Structural analysis of the extracellular polysaccharide produced by Sphaerotilus natans. | |
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MedLine Citation:
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PMID: 12224639 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An extracellular polysaccharide (EPS) was recovered and purified from the culture fluid of a sheathed bacterium, Sphaerotilus natans. Glucose, rhamnose, and aldobiouronic acid were detected in the acid hydrolysate of EPS by thin-layer chromatography (TLC). The aldobiouronic acid was found to be composed of glucuronic acid and rhamnose by TLC and gas-liquid chromatography analyses of the corresponding neutral disaccharide. The structure of EPS was identified by methylation linkage analysis and nuclear magnetic resonance. Additionally, partial acid hydrolysates of EPS were prepared and put through fast atom bombardment-mass spectrometry to determine the sugar sequence of EPS. The resulting data showed that EPS produced by S. natans is a new gellan-like polysaccharide constructed from a tetrasaccharide repeating unit, as shown below. -->4)-alpha-D-Glcp-(1-->2)-beta-D-GlcA p-(1-->2)-alpha-L-Rha p-(1-->3)-beta-L-Rha p-(1-->. |
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Authors:
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Minoru Takeda; Shogo Nomoto; Jun-ichi Koizumi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 66 ISSN: 0916-8451 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2002 Jul |
Date Detail:
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Created Date: 2002-09-12 Completed Date: 2003-03-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 1546-51 Citation Subset: IM |
Affiliation:
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Division of Materials Science and Chemical Engineering,Faculty of Engineering, Yokohama National University, Kanagawa, Japan. mtake@ynu.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carbohydrates
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chemistry Chromatography, Gas Gram-Negative Aerobic Bacteria / chemistry, metabolism* Magnetic Resonance Spectroscopy Methylation Polysaccharides / chemistry, metabolism* Spectrometry, Mass, Fast Atom Bombardment Uronic Acids / analysis |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Polysaccharides; 0/Uronic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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