Document Detail

Structural analysis of the extracellular polysaccharide produced by Sphaerotilus natans.
MedLine Citation:
PMID:  12224639     Owner:  NLM     Status:  MEDLINE    
An extracellular polysaccharide (EPS) was recovered and purified from the culture fluid of a sheathed bacterium, Sphaerotilus natans. Glucose, rhamnose, and aldobiouronic acid were detected in the acid hydrolysate of EPS by thin-layer chromatography (TLC). The aldobiouronic acid was found to be composed of glucuronic acid and rhamnose by TLC and gas-liquid chromatography analyses of the corresponding neutral disaccharide. The structure of EPS was identified by methylation linkage analysis and nuclear magnetic resonance. Additionally, partial acid hydrolysates of EPS were prepared and put through fast atom bombardment-mass spectrometry to determine the sugar sequence of EPS. The resulting data showed that EPS produced by S. natans is a new gellan-like polysaccharide constructed from a tetrasaccharide repeating unit, as shown below. -->4)-alpha-D-Glcp-(1-->2)-beta-D-GlcA p-(1-->2)-alpha-L-Rha p-(1-->3)-beta-L-Rha p-(1-->.
Minoru Takeda; Shogo Nomoto; Jun-ichi Koizumi
Related Documents :
1952059 - Measurement of uronic acids without interference from neutral sugars.
11117329 - Full structure of the o-specific polysaccharide of proteus mirabilis o24 containing 3,4...
9481879 - Nmr spectroscopic elucidation of the structure of a glycerol teichoic acid-like o-speci...
23292969 - Muehrcke's lines (leukonychia striata) due to transretinoic acid therapy for acute prom...
23079069 - Gout: thoughts about a treat-to-target programme.
3113949 - Characterization of a polysaccharide component of lipopolysaccharide from pseudomonas a...
2758029 - Further studies on the mechanism of inhibition of intestinal chylomicron transport by p...
16869989 - Absorption and metabolism of caffeic acid and chlorogenic acid in the small intestine o...
3159389 - Effects of diazinon on nucleotide and amino acid contents of chick embryos. teratogenic...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  66     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-09-12     Completed Date:  2003-03-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1546-51     Citation Subset:  IM    
Division of Materials Science and Chemical Engineering,Faculty of Engineering, Yokohama National University, Kanagawa, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Carbohydrates / chemistry
Chromatography, Gas
Gram-Negative Aerobic Bacteria / chemistry,  metabolism*
Magnetic Resonance Spectroscopy
Polysaccharides / chemistry,  metabolism*
Spectrometry, Mass, Fast Atom Bombardment
Uronic Acids / analysis
Reg. No./Substance:
0/Carbohydrates; 0/Polysaccharides; 0/Uronic Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Significance of the 20-kDa subunit of heterodimeric 2-deoxy-scyllo-inosose synthase for the biosynth...
Next Document:  Alpha-glucosidase inhibitory activity of a 70% methanol extract from ezoishige (Pelvetia babingtonii...