Document Detail

Structural analysis of the extracellular polysaccharide produced by Sphaerotilus natans.
MedLine Citation:
PMID:  12224639     Owner:  NLM     Status:  MEDLINE    
An extracellular polysaccharide (EPS) was recovered and purified from the culture fluid of a sheathed bacterium, Sphaerotilus natans. Glucose, rhamnose, and aldobiouronic acid were detected in the acid hydrolysate of EPS by thin-layer chromatography (TLC). The aldobiouronic acid was found to be composed of glucuronic acid and rhamnose by TLC and gas-liquid chromatography analyses of the corresponding neutral disaccharide. The structure of EPS was identified by methylation linkage analysis and nuclear magnetic resonance. Additionally, partial acid hydrolysates of EPS were prepared and put through fast atom bombardment-mass spectrometry to determine the sugar sequence of EPS. The resulting data showed that EPS produced by S. natans is a new gellan-like polysaccharide constructed from a tetrasaccharide repeating unit, as shown below. -->4)-alpha-D-Glcp-(1-->2)-beta-D-GlcA p-(1-->2)-alpha-L-Rha p-(1-->3)-beta-L-Rha p-(1-->.
Minoru Takeda; Shogo Nomoto; Jun-ichi Koizumi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  66     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-09-12     Completed Date:  2003-03-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1546-51     Citation Subset:  IM    
Division of Materials Science and Chemical Engineering,Faculty of Engineering, Yokohama National University, Kanagawa, Japan.
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MeSH Terms
Carbohydrates / chemistry
Chromatography, Gas
Gram-Negative Aerobic Bacteria / chemistry,  metabolism*
Magnetic Resonance Spectroscopy
Polysaccharides / chemistry,  metabolism*
Spectrometry, Mass, Fast Atom Bombardment
Uronic Acids / analysis
Reg. No./Substance:
0/Carbohydrates; 0/Polysaccharides; 0/Uronic Acids

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