| Structural analysis and classification of native proteins from E. coli commonly co-purified by immobilised metal affinity chromatography. | |
| | |
MedLine Citation:
|
PMID: 16814929 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Immobilised metal affinity chromatography (IMAC) is the most widely used technique for single-step purification of recombinant proteins. However, despite its use in the purification of heterologue proteins in the eubacteria Escherichia coli for decades, the presence of native E. coli proteins that exhibit a high affinity for divalent cations such as nickel, cobalt or copper has remained problematic. This is of particular relevance when recombinant molecules are not expressed at high levels or when their overexpression induces that of native bacterial proteins due to pleiotropism and/or in response to stress conditions. Identification of such contaminating proteins is clearly relevant to those involved in the purification of histidine-tagged proteins either at small/medium scale or in high-throughput processes. The work presented here reviews the native proteins from E. coli most commonly co-purified by IMAC, including Fur, Crp, ArgE, SlyD, GlmS, GlgA, ODO1, ODO2, YadF and YfbG. The binding of these proteins to metal-chelating resins can mostly be explained by their native metal-binding functions or their possession of surface clusters of histidine residues. However, some proteins fall outside these categories, implying that a further class of interactions may account for their ability to co-purify with histidine-tagged proteins. We propose a classification of these E. coli native proteins based on their physicochemical, structural and functional properties. |
| | |
Authors:
|
Victor Martin Bolanos-Garcia; Owen Richard Davies |
Related Documents
:
|
21439479 - Context-based identification of protein-protein interfaces and "hot-spot" residues. 21448279 - Detection of prion protein in urine-derived injectable fertility products by a targeted... 12821199 - Two-step purification of his-tagged nef protein in native condition using heparin and i... 3782369 - Comparison of reversed-phase column materials for high-performance liquid chromatograph... 18273849 - A robust protein host for anchoring chelating ligands and organocatalysts. 11388809 - Overexpression, rapid isolation, and biochemical characterization of escherichia coli s... 19398759 - Active establishment of centromeric cenp-a chromatin by rsf complex. 12744289 - Studies on the amino acid and mineral content of buckwheat protein fractions. 1998709 - Long-term intercalation of residual hemin in erythrocyte membranes distorts the cell. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Review Date: 2006-04-26 |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 1760 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2006 Sep |
Date Detail:
|
Created Date: 2006-09-18 Completed Date: 2006-11-14 Revised Date: 2007-08-13 |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 1304-13 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, England. victor@cryst.bioc.cam.ac.uk |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Chromatography, Affinity Escherichia coli / chemistry*, genetics, metabolism* Escherichia coli Proteins / chemistry*, classification, isolation & purification, metabolism* Humans Metals / chemistry*, metabolism* Models, Molecular Protein Binding |
| Grant Support | |
ID/Acronym/Agency:
|
//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
|
0/Escherichia coli Proteins; 0/Metals |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Diagnostic value of MRI in a pelvic mass of prostatic origin.
Next Document: Clozapine reverses increased brown adipose tissue thermogenesis induced by 3,4-methylenedioxymethamp...