Document Detail


Structural analysis of the carbohydrate components of the outer membrane of the lipopolysaccharide-lacking cellulolytic ruminal bacterium Fibrobacter succinogenes S85.
MedLine Citation:
PMID:  11422388     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The polysaccharides from the outer membrane of the Gram-negative ruminal bacterium Fibrobacter succinogenes were isolated by phenol/water extraction and separated by size-exclusion chromatography in the presence of deoxycholate detergent into a lower-molecular-mass fraction designated 'glycolipid' and a high-molecular-mass 'capsular polysaccharide' fraction. Both fractions lacked typical lipopolysaccharide components including 2-keto-3-deoxyoctulosonic acid and 3-hydroxy fatty acids. Carbohydrate components of these fractions were represented by two polysaccharides and one oligosaccharide (possibly glycolipid) with the following structures: : : where HEAEP is N-(2-hydroxyethyl)-2-aminoethylphosphonic acid, found for the first time in natural compounds. The polysaccharides contained pentadecanoic acid and anteisopentadecanoic acid, possibly present as the acyl components. All constituent monosaccharides except L-rhamnose had a D-configuration. In addition to having a structural role in the outer membrane, these polysaccharides may provide protection for this lipopolysaccharide-less bacterium in the highly competitive ruminal environment, as phosphonic acids covalently linked to membrane polymers have in the past been attributed the function of stabilizing membranes in the presence of phosphatases and lipases.
Authors:
E Vinogradov; E E Egbosimba; M B Perry; J S Lam; C W Forsberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  268     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-06-25     Completed Date:  2001-08-02     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  3566-76     Citation Subset:  IM    
Affiliation:
Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada. evguenii@vinogradov@nrc.ca
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteria / metabolism*
Carbohydrate Conformation
Carbohydrate Metabolism*
Carbohydrate Sequence
Carbohydrates / chemistry
Cellulose / metabolism*
Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
Lipopolysaccharides / chemistry,  metabolism*
Molecular Sequence Data
Rumen / microbiology*
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Lipopolysaccharides; 9004-34-6/Cellulose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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