| Structural analysis of Bacillus megaterium KM spore peptidoglycan and its dynamics during germination. | |
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MedLine Citation:
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PMID: 10376818 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The composition and structure of peptidoglycan from dormant spores of Bacillus megaterium KM and its dynamics during germination were investigated. Amino acid analysis and mass spectrometry identified 21 muropeptides resolved by reverse phase HPLC following digestion of peptidoglycan with Cellosyl. The basic structure of peptidoglycan in B. megaterium spores is similar to that of Bacillus subtilis: 44.2% of muramic acid residues are substituted with delta-lactam, 28.8% with single L-alanine, 25.1% with tetrapeptide and only 1.8% with tripeptide. The cross-linking index of the spore peptidoglycan, determined from muropeptides resolved by reverse phase HPLC, was 2.2 % per muramic acid. Spore peptidoglycan contains 2.9% of muropeptides with unsubstituted N-acetylmuramic acid. These muropeptides are likely to be intermediate products of delta-lactam formation. Analysis of muropeptide dynamics during germination revealed the activity of at least two hydrolytic enzymes, an N-acetylglucosaminidase and a lytic transglycosylase. A 4 M LiCl extract from 30 min germinated spores of B. megaterium KM caused 'germination-like' changes to permeabilized spores of B. megaterium and B. subtilis but not those of a B. subtilis cwlD mutant. Muropeptide analysis of the treated spores revealed the presence of products generated by the activity of a glucosaminidase. |
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Authors:
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A Atrih; G Bacher; R Körner; G Allmaier; S J Foster |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Microbiology (Reading, England) Volume: 145 ( Pt 5) ISSN: 1350-0872 ISO Abbreviation: Microbiology (Reading, Engl.) Publication Date: 1999 May |
Date Detail:
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Created Date: 1999-09-21 Completed Date: 1999-09-21 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9430468 Medline TA: Microbiology Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1033-41 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology and Biotechnology, University of Sheffield, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylglucosaminidase
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metabolism Amino Acids / analysis Bacillus megaterium / physiology* Chromatography, High Pressure Liquid Ions Mass Spectrometry Muramidase / metabolism Peptidoglycan / chemistry*, metabolism* Spores, Bacterial / physiology* Structure-Activity Relationship |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Ions; 0/Peptidoglycan; EC 3.2.1.17/Muramidase; EC 3.2.1.52/Acetylglucosaminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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