Document Detail

Structural analysis of 3-isopropylmalate dehydrogenase from the obligate piezophile Shewanella benthica DB21MT-2 and the nonpiezophile Shewanella oneidensis MR-1.
MedLine Citation:
PMID:  22442218     Owner:  NLM     Status:  MEDLINE    
Organisms living in deep seas such as the Mariana Trench must be adapted to the extremely high pressure environment. For example, the 3-isopropylmalate dehydrogenase from the obligate piezophile Shewanella benthica DB21MT-2 (SbIPMDH) remains active in extreme conditions under which that from the land bacterium S. oneidensis MR-1 (SoIPMDH) becomes inactivated. In order to unravel the differences between these two IPMDHs, their structures were determined at ~1.5 Å resolution. Comparison of the structures of the two enzymes shows that SbIPMDH is in a more open form and has a larger internal cavity volume than SoIPMDH at atmospheric pressure. This loosely packed structure of SbIPMDH could help it to avoid pressure-induced distortion of the native structure and to remain active at higher pressures than SoIPMDH.
Takayuki Nagae; Chiaki Kato; Nobuhisa Watanabe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-02-15
Journal Detail:
Title:  Acta crystallographica. Section F, Structural biology and crystallization communications     Volume:  68     ISSN:  1744-3091     ISO Abbreviation:  Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-23     Completed Date:  2012-05-11     Revised Date:  2014-03-12    
Medline Journal Info:
Nlm Unique ID:  101226117     Medline TA:  Acta Crystallogr Sect F Struct Biol Cryst Commun     Country:  England    
Other Details:
Languages:  eng     Pagination:  265-8     Citation Subset:  IM    
Copyright Information:
© 2012 International Union of Crystallography
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MeSH Terms
3-Isopropylmalate Dehydrogenase / chemistry*
Models, Molecular
Protein Structure, Quaternary
Protein Structure, Tertiary
Shewanella / enzymology*
Structural Homology, Protein
Reg. No./Substance:
EC Dehydrogenase

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