| Structural analysis of 3-isopropylmalate dehydrogenase from the obligate piezophile Shewanella benthica DB21MT-2 and the nonpiezophile Shewanella oneidensis MR-1. | |
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MedLine Citation:
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PMID: 22442218 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Organisms living in deep seas such as the Mariana Trench must be adapted to the extremely high pressure environment. For example, the 3-isopropylmalate dehydrogenase from the obligate piezophile Shewanella benthica DB21MT-2 (SbIPMDH) remains active in extreme conditions under which that from the land bacterium S. oneidensis MR-1 (SoIPMDH) becomes inactivated. In order to unravel the differences between these two IPMDHs, their structures were determined at ∼1.5 Å resolution. Comparison of the structures of the two enzymes shows that SbIPMDH is in a more open form and has a larger internal cavity volume than SoIPMDH at atmospheric pressure. This loosely packed structure of SbIPMDH could help it to avoid pressure-induced distortion of the native structure and to remain active at higher pressures than SoIPMDH. |
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Authors:
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Takayuki Nagae; Chiaki Kato; Nobuhisa Watanabe |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-2-15 |
Journal Detail:
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Title: Acta crystallographica. Section F, Structural biology and crystallization communications Volume: 68 ISSN: 1744-3091 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101226117 Medline TA: Acta Crystallogr Sect F Struct Biol Cryst Commun Country: - |
Other Details:
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Languages: ENG Pagination: 265-268 Citation Subset: - |
Affiliation:
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Department of Biotechnology, Graduate School of Engineering, Nagoya University, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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