| Structural analyses of the lipopolysaccharides from Chlamydophila psittaci strain 6BC and Chlamydophila pneumoniae strain Kajaani 6. | |
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MedLine Citation:
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PMID: 11705470 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lipopolysaccharides (LPSs) of Chlamydophila psittaci 6BC and Chlamydophila pneumoniae Kajaani 6 contain 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo), GlcN, organic bound phosphate, and fatty acids in the molar ratios of approximately 3:2:2.2:4.8 and approximately 2.9:2:2.1:4.9, respectively. The LPSs were immunoreactive with a monoclonal antibody against a family-specific epitope of chlamydial LPS. This finding, together with methylation analyses of both LPSs and MALDI-TOF MS experiments on de-O-, and de-O,N-acylated LPSs, indicate the presence of a Kdo trisaccharide proximal to lipid A having a structure alpha-Kdo-(2-->8)-alpha-Kdo-(2-->4)-alpha-Kdo, which appears to be the main component of the core region in the native chlamydial LPSs. In the de-O-acylated LPSs from Chl. psittaci 6BC and Chl. pneumoniae Kajaani 6, two major molecular species are present that differ in distribution of amide-bound hydroxy fatty acids over both GlcN. It appears that either two (R)-3-hydroxy-18-methylicosanoic acids or one (R)-3-hydroxy-18-methylicosanoic acid and one (R)-3-hydroxyicosanoic acid are attached to the GlcN residues. In contrast, the de-O-acylated LPS of Chl. psittaci PK 5082 contains one major molecular species that has two (R)-3-hydroxyicosanoic acid residues attached to two GlcN residues. |
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Authors:
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A Hussein; L Skultéty; R Toman |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Carbohydrate research Volume: 336 ISSN: 0008-6215 ISO Abbreviation: Carbohydr. Res. Publication Date: 2001 Nov |
Date Detail:
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Created Date: 2001-11-13 Completed Date: 2002-03-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0043535 Medline TA: Carbohydr Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 213-23 Citation Subset: IM |
Affiliation:
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Department of Rickettsiology and Chlamydiology, Institute of Virology, Slovak Academy of Sciences, Dúbravská cesta 9, 842 45 Bratislava, Slovak Republic. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Western Carbohydrate Sequence Chlamydophila pneumoniae / chemistry* Chlamydophila psittaci / chemistry* Fatty Acids / analysis Lipopolysaccharides / analysis, chemistry* Molecular Sequence Data Molecular Structure Phosphoric Acid Esters / analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sugar Acids / analysis |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Lipopolysaccharides; 0/Phosphoric Acid Esters; 0/Sugar Acids; 1069-03-0/2-keto-3-deoxyoctonate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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