Document Detail


Structural analyses of the lipopolysaccharides from Chlamydophila psittaci strain 6BC and Chlamydophila pneumoniae strain Kajaani 6.
MedLine Citation:
PMID:  11705470     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipopolysaccharides (LPSs) of Chlamydophila psittaci 6BC and Chlamydophila pneumoniae Kajaani 6 contain 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo), GlcN, organic bound phosphate, and fatty acids in the molar ratios of approximately 3:2:2.2:4.8 and approximately 2.9:2:2.1:4.9, respectively. The LPSs were immunoreactive with a monoclonal antibody against a family-specific epitope of chlamydial LPS. This finding, together with methylation analyses of both LPSs and MALDI-TOF MS experiments on de-O-, and de-O,N-acylated LPSs, indicate the presence of a Kdo trisaccharide proximal to lipid A having a structure alpha-Kdo-(2-->8)-alpha-Kdo-(2-->4)-alpha-Kdo, which appears to be the main component of the core region in the native chlamydial LPSs. In the de-O-acylated LPSs from Chl. psittaci 6BC and Chl. pneumoniae Kajaani 6, two major molecular species are present that differ in distribution of amide-bound hydroxy fatty acids over both GlcN. It appears that either two (R)-3-hydroxy-18-methylicosanoic acids or one (R)-3-hydroxy-18-methylicosanoic acid and one (R)-3-hydroxyicosanoic acid are attached to the GlcN residues. In contrast, the de-O-acylated LPS of Chl. psittaci PK 5082 contains one major molecular species that has two (R)-3-hydroxyicosanoic acid residues attached to two GlcN residues.
Authors:
A Hussein; L Skultéty; R Toman
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Carbohydrate research     Volume:  336     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-11-13     Completed Date:  2002-03-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  213-23     Citation Subset:  IM    
Affiliation:
Department of Rickettsiology and Chlamydiology, Institute of Virology, Slovak Academy of Sciences, Dúbravská cesta 9, 842 45 Bratislava, Slovak Republic.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Carbohydrate Sequence
Chlamydophila pneumoniae / chemistry*
Chlamydophila psittaci / chemistry*
Fatty Acids / analysis
Lipopolysaccharides / analysis,  chemistry*
Molecular Sequence Data
Molecular Structure
Phosphoric Acid Esters / analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Sugar Acids / analysis
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Lipopolysaccharides; 0/Phosphoric Acid Esters; 0/Sugar Acids; 1069-03-0/2-keto-3-deoxyoctonate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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