Document Detail


Structural alterations of the mitotic apparatus induced by the heat shock response in Drosophila cells.
MedLine Citation:
PMID:  9297784     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The general architecture of the mitotic apparatus was studied at the ultrastructural level in Drosophila cultured cells. Its two main characteristics are a very polarized spindle and a strong compartmentalization, ensured by large remnants of the nuclear envelope. Such compartmentalization has previously been reported for the rapid syncytial divisions of the early embryo; a similar finding in these cells with a long cycle strongly suggests that this organization constitutes a general mechanism for mitosis in Drosophila. We followed the modifications of these structures after a heat shock of 20, 50 or 120 min at 37 degrees C. Contrary to interphase cells, mitotic cells appear very sensitive to hyperthermia. This stress treatment induced a disruption of the mitotic spindle, a reappearance and an extension of the Golgi apparatus, an inactivation of microtubule nucleation and a disorganization of the centrosome. This organelle seems the first to be affected by the heat shock response. The centrosome is not only inactivated, but also is structurally affected. During the recovery phase after heat stress, the mitotic cells presented a remarkable ring-shaped accumulation of electron-dense material around the centrioles. We conclude that in Drosophila cells the mitotic phase, and more specifically the centrosome, are targets of the stress response.
Authors:
A Debec; C Marcaillou
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biology of the cell / under the auspices of the European Cell Biology Organization     Volume:  89     ISSN:  0248-4900     ISO Abbreviation:  Biol. Cell     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-10-23     Completed Date:  1997-10-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8108529     Medline TA:  Biol Cell     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  67-78     Citation Subset:  IM    
Affiliation:
Laboratoire de Physiologie Cellulaire des Invertébrés, Université Pierre-et-Marie-Curie, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Centrioles / physiology,  ultrastructure
Centrosome / physiology,  ultrastructure
Drosophila
Heat-Shock Proteins / physiology*
Hot Temperature
Microscopy, Electron
Microtubules / physiology,  ultrastructure
Mitosis / physiology*
Stress, Physiological / physiopathology
Chemical
Reg. No./Substance:
0/Heat-Shock Proteins

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