| Structural alterations of erythrocyte membrane components induced by exhaustive exercise. | |
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MedLine Citation:
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PMID: 19088781 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Physical exercise was used as a model of the physiological modulator of free radical production to examine the effects of exercise-induced oxidative modifications on the physico-biochemical properties of erythrocyte membrane. The aim of our work was to investigate conformational changes of erythrocyte membrane proteins, membrane fluidity, and membrane susceptibility to disintegration. Venous blood was taken before, immediately after, and 1 h after an exhaustive incremental cycling test (30 W.min-1 ramp), performed by 11 healthy untrained males on balanced diets (mean age, 22 +/- 2 years; mean body mass index, 25 +/- 4.5 kg.m-2). In response to this exercise, individual maximum heart rate was 195 +/- 12 beats.min-1 and maximum wattage was 292 +/- 27 W. Electron paramagnetic resonance spectroscopy was used to investigate alterations in membrane proteins and membrane dynamics, and to measure production of radical species. The reducing potential of plasma (RPP) was measured using the reduction of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the ferric-reducing ability of plasma. Exercise induced decreases in erythrocyte membrane fluidity in the polar region (p < 0.0001) and alterations in the conformational state of membrane proteins (p < 0.05). An increase in RPP was observed immediately after exercise (p < 0.001), with a further increase 1 h postexercise (p < 0.0001). Supporting measurements of lipid peroxidation showed an increase in thiobarbituric acid reactive substances immediately after exercise (p < 0.05) and at 1 h of recovery (p < 0.001); however, free radicals were not detected. Results indicate the existence of early postexercise mild oxidative stress after single-exercise performance, which induced structural changes in erythrocyte membrane components (protein aggregation) and in the membrane organization (lipids rigidization) that followed lipid peroxidation but did not lead to cellular hemolysis. |
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Authors:
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Joanna Brzeszczynska; Anna Pieniazek; Lukasz Gwozdzinski; Krzysztof Gwozdzinski; Anna Jegier |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied physiology, nutrition, and metabolism = Physiologie appliquée, nutrition et métabolisme Volume: 33 ISSN: 1715-5312 ISO Abbreviation: - Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-12-17 Completed Date: 2009-03-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101264333 Medline TA: Appl Physiol Nutr Metab Country: Canada |
Other Details:
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Languages: eng Pagination: 1223-31 Citation Subset: IM |
Affiliation:
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School of Life Sciences, Heriot-Watt University, Edinburgh, EH14 4AS, UK. Joanna.Brzeszczynska@hw.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Analysis of Variance Bicycling Biological Markers / blood Electron Spin Resonance Spectroscopy / methods Erythrocyte Membrane / physiology* Exercise / physiology* Heart Rate / physiology Humans Lipid Peroxidation / physiology Male Membrane Fluidity / physiology Membrane Proteins / blood, physiology Osmotic Fragility / physiology Physical Endurance / physiology* Physical Exertion / physiology Reactive Oxygen Species / blood Reference Values Spin Labels Spin Trapping / methods Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Membrane Proteins; 0/Reactive Oxygen Species; 0/Spin Labels |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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