Document Detail


Structural typing of systemic amyloidoses by luminescent-conjugated polymer spectroscopy.
MedLine Citation:
PMID:  20035056     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Most systemic amyloidoses are progressive and lethal, and their therapy depends on the identification of the offending proteins. Here we report that luminescent-conjugated thiophene polymers (LCP) sensitively detect amyloid deposits. The heterodisperse polythiophene acetic acid derivatives, polythiophene acetic acid (PTAA) and trimeric PTAA, emitted yellow-red fluorescence on binding to amyloid deposits, whereas chemically homogeneous pentameric formic thiophene acetic acid emitted green-yellow fluorescence. The geometry of LCPs modulates the spectral composition of the emitted light, thereby reporting ligand-induced steric changes. Accordingly, a screen of PTAA-stained amyloid deposits in histological tissue arrays revealed striking spectral differences between specimens. Blinded cluster assignments of spectral profiles of tissue samples from 108 tissue samples derived from 96 patients identified three nonoverlapping classes, which were found to match AA, AL, and ATTR immunotyping. We conclude that LCP spectroscopy is a sensitive and powerful tool for identifying and characterizing amyloid deposits.
Authors:
K Peter R Nilsson; Kristian Ikenberg; Andreas Aslund; Sophia Fransson; Peter Konradsson; Christoph Röcken; Holger Moch; Adriano Aguzzi
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-24
Journal Detail:
Title:  The American journal of pathology     Volume:  176     ISSN:  1525-2191     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-26     Completed Date:  2010-03-16     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  563-74     Citation Subset:  AIM; IM    
Affiliation:
Institute of Neuropathology, Department of Pathology, University Hospital of Zurich,CH-8091 Zurich, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Acetic Acid / chemistry
Adolescent
Adult
Aged
Aged, 80 and over
Amyloid / chemistry*,  classification*,  metabolism
Amyloidosis / metabolism*,  pathology*
Female
Humans
Luminescent Agents / chemistry,  pharmacology*
Male
Middle Aged
Molecular Structure
Polymers / chemistry,  pharmacology*
Sensitivity and Specificity
Spectrometry, Fluorescence / methods
Staining and Labeling / methods
Thiophenes / chemistry,  pharmacology
Young Adult
Chemical
Reg. No./Substance:
0/Amyloid; 0/Luminescent Agents; 0/Polymers; 0/Thiophenes; 25233-34-5/polythiophene; 64-19-7/Acetic Acid
Comments/Corrections

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