Document Detail

Structural and Dynamic Features of Candida rugosa Lipase1 in Water, Octane, Toluene and Ionic Liquids BMIM-PF6 and BMIM-NO3.
MedLine Citation:
PMID:  23387335     Owner:  NLM     Status:  Publisher    
Ionic liquids (ILs) and organic chemicals can be used as solvents in biochemical reactions to influence the structural and dynamic features of the enzyme, sometimes detrimentally. In this work we report the results for molecular dynamics simulations of Candida rugosa lipase (CRL) in ILs BMIM-PF6 and BMIM-NO3, as well as organic solvents toluene and octane in an effort to explore the role of solvent on the structure and dynamics of an enzyme known to be active in many non-aqueous media. Simulations of CRL in water were also included for comparison, bringing the aggregate simulation time to over 2.8 μs. At both 310 and 375 K the ILs significantly dampen protein dynamics and trap the system near its starting structure. Structural changes in the enzyme follow the viscosity of the solvent, with the enzyme deviating from its initial structure the most in water and the least in BMIM-PF6. Interactions between the enzyme surface and the solvent in the IL simulations show that contacts are dominated by the IL anion, which is ascribed to a broader spatial distribution of positively charged protein residues and reduced mobility of the cation due to the size of the imadazolium ring.
Patrick R Burney; Jim Pfaendtner
Related Documents :
24158265 - Electro-hydrodynamic instability of stressed viscoelastic polymer films.
23135675 - Regulation of hard α-keratin mechanics via control of intermediate filament hydration:...
1619565 - Influence of d-glucose-induced water absorption on rat jejunal uptake of two passively ...
20873705 - Laboratory measurement of the absorption coefficient of riboflavin for ultraviolet ligh...
16677665 - Steady electro-optic characteristics of noninteracting colloidal particles.
15623315 - Radio frequency plasma polymer coatings for affinity capture maldi mass spectrometry.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-6
Journal Detail:
Title:  The journal of physical chemistry. B     Volume:  -     ISSN:  1520-5207     ISO Abbreviation:  J Phys Chem B     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101157530     Medline TA:  J Phys Chem B     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Treatment of Hemi-Mandibulectomy Defect with Implant-Supported Telescopic Removable Prosthesis. A Cl...
Next Document:  Toll-like receptor 3 activation in retinal pigment epithelium cells - Mitogen-activated protein kina...