| Structural determination of a key exopolysaccharide in mixed culture aerobic sludge granules using NMR spectroscopy. | |
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MedLine Citation:
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PMID: 21033741 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Nuclear magnetic resonance (NMR) techniques were used to elucidate the structure of an exopolysaccharide material previously revealed to be important in formation of aerobic granules. The 1D NMR spectral data acquired showed that this gel-forming polysaccharide was a major component of granular EPS, while 1D and 2D NMR spectra showed it consisted of eight sugar residues. These were assigned as α-galactose, α-rhamnose, 2-acetoamido-2-deoxy-α-galactopyranuronic acid, β-mannose, β-galactose, β-glucuronate, β-glucosamine, and N-acetyl β-galactosamine. With the exception of 2-acetoamido-2-deoxy-α-galactopyranuronic acid, a highly unusual sugar, their presence was confirmed with high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Carbon and proton shifts were assigned for each sugar. Heteronuclear multiple bond correlation (HMBC) and nuclear Overhauser enhancement spectroscopy (NOESY) were used to identify linkage sites between individual sugar residues. This gel-forming exopolysaccharide appeared to be a highly complex single heteropolysaccharide with a repeat sequence of α-galactose, β-mannose, β-glucosamine, N-acetyl-β-galactosamine, and 2-acetoamido-2-deoxy-α-galactopyranuronic acid. It has a disaccharide branch of β-galactose and β-glucuronic acid attached to 2-acetoamido-2-deoxy-α-galactopyranuronic acid and an α-rhamnose branch attached to α-galactose. |
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Authors:
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Thomas Seviour; Lynette K Lambert; Maite Pijuan; Zhiguo Yuan |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-29 |
Journal Detail:
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Title: Environmental science & technology Volume: 44 ISSN: 1520-5851 ISO Abbreviation: Environ. Sci. Technol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0213155 Medline TA: Environ Sci Technol Country: United States |
Other Details:
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Languages: eng Pagination: 8964-70 Citation Subset: IM |
Affiliation:
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The University of Queensland, Advanced Water Management Centre, St. Lucia, QLD 4072, Australia. |
Export Citation:
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Descriptor/Qualifier:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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