Document Detail


Structural basis of efficient electron transport between photosynthetic membrane proteins and plastocyanin in spinach revealed using nuclear magnetic resonance.
MedLine Citation:
PMID:  23032988     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the photosynthetic light reactions of plants and cyanobacteria, plastocyanin (Pc) plays a crucial role as an electron carrier and shuttle protein between two membrane protein complexes: cytochrome b(6)f (cyt b(6)f) and photosystem I (PSI). The rapid turnover of Pc between cyt b(6)f and PSI enables the efficient use of light energy. In the Pc-cyt b(6)f and Pc-PSI electron transfer complexes, the electron transfer reactions are accomplished within <10(-4) s. However, the mechanisms enabling the rapid association and dissociation of Pc are still unclear because of the lack of an appropriate method to study huge complexes with short lifetimes. Here, using the transferred cross-saturation method, we investigated the residues of spinach (Spinacia oleracea) Pc in close proximity to spinach PSI and cyt b(6)f, in both the thylakoid vesicle-embedded and solubilized states. We demonstrated that the hydrophobic patch residues of Pc are in close proximity to PSI and cyt b(6)f, whereas the acidic patch residues of Pc do not form stable salt bridges with either PSI or cyt b(6)f, in the electron transfer complexes. The transient characteristics of the interactions on the acidic patch facilitate the rapid association and dissociation of Pc.
Authors:
Takumi Ueda; Naoko Nomoto; Masamichi Koga; Hiroki Ogasa; Yuuta Ogawa; Masahiko Matsumoto; Pavlos Stampoulis; Koji Sode; Hiroaki Terasawa; Ichio Shimada
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-02
Journal Detail:
Title:  The Plant cell     Volume:  24     ISSN:  1532-298X     ISO Abbreviation:  Plant Cell     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-12-05     Completed Date:  2013-06-20     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4173-86     Citation Subset:  IM    
Affiliation:
Graduate School of Pharmaceutical Sciences, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/X04693; PDB/1CTM;  2O01; SWISSPROT/Q09099
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MeSH Terms
Descriptor/Qualifier:
Cytochrome b6f Complex / metabolism
Electron Transport
Hydrophobic and Hydrophilic Interactions
Magnetic Resonance Spectroscopy
Membrane Proteins / chemistry,  metabolism*
Molecular Sequence Data
Photosynthesis
Photosystem I Protein Complex / metabolism
Plastocyanin / chemistry,  metabolism*
Spinacia oleracea / metabolism*
Chemical
Reg. No./Substance:
0/Membrane Proteins; 0/Photosystem I Protein Complex; 9014-09-9/Plastocyanin; 9035-40-9/Cytochrome b6f Complex
Comments/Corrections

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