Document Detail


Structural alterations of the FAS gene in cutaneous T-cell lymphoma (CTCL).
MedLine Citation:
PMID:  21036138     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
FAS (TNF receptor superfamily member 6, also known as CD95) plays a major role in T-cell apoptosis and is often dysregulated in CTCL. We searched for structural alterations of the FAS gene with the potential to affect its function. Although several heterozygous FAS promoter single nucleotide polymorphisms (SNPs) were detected, the only homozygous one was the -671 GG SNP present in 24/80 CTCL cases (30%). This SNP maps to an interferon response element activated by STAT-1. EMSA and supershift EMSA showed decreased CTCL nuclear protein/STAT-1 binding to oligonucleotides bearing this SNP. Luciferase reporters showed significantly less interferon-alfa responsive expression by FAS promoter constructs containing this SNP in multiple CTCL lines. Finally, FAS was upregulated by interferon-alfa in wildtype CTCL cells but not those bearing the -671 GG SNP. These findings indicate that many CTCL patients harbor the homozygous FAS promoter -671 GG SNP capable of blunting its response to interferon. This may have implications for CTCL pathogenesis, racial incidence and the response of patients to interferon-alfa therapy. In contrast, functionally significant mutations in FAS coding sequences were detected uncommonly. Among CTCL lines with the potential to serve as models of FAS regulation, FAS-high MyLa had both FAS alleles, FAS-low HH was FAS-hemizygous and FAS-negative SeAx was FAS-null.
Authors:
Jianqiang Wu; Jawed Siddiqui; Minakshi Nihal; Eric C Vonderheid; Gary S Wood
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-10-29
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  508     ISSN:  1096-0384     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-18     Completed Date:  2011-05-18     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  185-91     Citation Subset:  IM    
Copyright Information:
Published by Elsevier Inc.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD95 / genetics*
Cell Line
Chromosome Aberrations / drug effects
Chromosomes, Human, Pair 10 / genetics
Gene Expression Regulation, Neoplastic / drug effects
Genotype
Germ-Line Mutation / drug effects,  genetics
Humans
In Situ Hybridization, Fluorescence
Interferon-alpha / pharmacology
Lymphoma, T-Cell, Cutaneous / genetics*
Polymorphism, Single Nucleotide / drug effects
Promoter Regions, Genetic / genetics
STAT1 Transcription Factor / metabolism
Skin Neoplasms / genetics*
Up-Regulation / drug effects
Grant Support
ID/Acronym/Agency:
1UL1RR025011/RR/NCRR NIH HHS; UL1 RR025011/RR/NCRR NIH HHS; UL1 RR025011-01/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD95; 0/Interferon-alpha; 0/STAT1 Transcription Factor
Comments/Corrections

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