| Strong immunoreactivity of cathepsin L at the site of rimmed vacuoles in diseased muscles. | |
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MedLine Citation:
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PMID: 1559157 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Skeletal muscle samples from four patients with myopathies showing autophagic vacuole formation were examined by immunohistochemical, biochemical and immunoblot analyses. Immunochemical studies demonstrated strongly positive reactions of cathepsins B and L in and around the rimmed vacuoles and weak reactions in intramyofibral portions of degenerating muscle fibres. Faint staining of cathepsin H was also seen at the sites of rimmed vacuoles. Biochemical analyses showed increased activity of cathepsin B and L in muscle specimens from the patients with rimmed vacuole formation. However, there was no remarkable cathepsin H activity in the muscle specimens from these patients. Cathepsin L immunoblot analysis of muscle extracts showed three bands with molecular masses of 30 kDa, 36 kDa and 39 kDa. The immunostaining of cathepsin L from patients with rimmed vacuole formation was stronger than that of normal controls. Cathepsin B immunoblot analysis showed only faint bands in samples from patients with rimmed vacuole formation and normal controls. This study demonstrated, for the first time, strong immunoreactivity of cathepsin L at the sites of rimmed vacuoles. Possible mechanisms of rimmed vacuole formation are discussed. |
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Authors:
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T Jimi; Y Satoh; A Takeda; S Shibuya; Y Wakayama; K Sugita |
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Publication Detail:
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Type: Case Reports; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Brain : a journal of neurology Volume: 115 Pt 1 ISSN: 0006-8950 ISO Abbreviation: Brain Publication Date: 1992 Feb |
Date Detail:
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Created Date: 1992-05-14 Completed Date: 1992-05-14 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372537 Medline TA: Brain Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 249-60 Citation Subset: AIM; IM |
Affiliation:
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Department of Medicine, Showa University Fujigaoka Hospital, Yokahama, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Cathepsin L Cathepsins / analysis* Cysteine Endopeptidases Endopeptidases* Female Humans Immunoblotting Immunohistochemistry Lysosomes / metabolism Male Middle Aged Muscular Diseases / metabolism* Vacuoles |
| Chemical | |
Reg. No./Substance:
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EC 3.4.-/Cathepsins; EC 3.4.-/Endopeptidases; EC 3.4.22.-/Cysteine Endopeptidases; EC 3.4.22.15/CTSL1 protein, human; EC 3.4.22.15/Cathepsin L |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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