Document Detail


Strong epigenetic similarity between maize centromeric and pericentromeric regions at the level of small RNAs, DNA methylation and H3 chromatin modifications.
MedLine Citation:
PMID:  22058126     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Both kinetochore function and sister chromatid cohesion can depend upon pericentromere chromatin structure, and factors associated with heterochromatin have been proposed to have general, conserved roles in distinguishing centromeres and pericentromeres and in conferring pericentromere-intrinsic functions. We applied genome-wide sequencing approaches to quantify RNA expression, DNA methylation and histone modification distributions in maize (Zea mays), focusing on two maize chromosomes with nearly fully sequenced centromeres and pericentromeres. Aside from the presence of the Histone H3 variant common to all centromeres, Centromeric Histone H3 (CENH3), we found no RNA expression or chromatin modifications that clearly differentiate pericentromeres from either centromeres or from chromosome arms, nor did we identify an epigenetic signature that accurately predicts CENH3 location. RNA expression and chromatin modification frequencies were broadly associated with distance from centromeres, gradually peaking or dipping toward arms. When interpreted in the context of experimental data from other systems, our results suggest that centromeres may confer essential functions (such as cohesion retention) to flanking sequence regardless of the local heterochromatin profile.
Authors:
Jonathan I Gent; Yuzhu Dong; Jiming Jiang; R Kelly Dawe
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-11-04
Journal Detail:
Title:  Nucleic acids research     Volume:  40     ISSN:  1362-4962     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-28     Completed Date:  2012-04-23     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  1550-60     Citation Subset:  IM    
Affiliation:
Department of Plant Biology, University of Georgia, Athens, Georgia 30602, USA.
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MeSH Terms
Descriptor/Qualifier:
Centromere / metabolism*
Chromatin / metabolism*
Chromosomes, Plant / metabolism
DNA Methylation*
Epigenesis, Genetic*
Euchromatin / metabolism
Histones / metabolism*
RNA, Messenger / metabolism
RNA, Small Interfering / metabolism*
Zea mays / genetics*,  metabolism
Grant Support
ID/Acronym/Agency:
0421671//PHS HHS; 0607123//PHS HHS; 0922703//PHS HHS; F32GM095223/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Chromatin; 0/Euchromatin; 0/Histones; 0/RNA, Messenger; 0/RNA, Small Interfering
Comments/Corrections

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