| Stromal-derived IL-6 alters the balance of myeloerythroid progenitors during Toxoplasma gondii infection. | |
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MedLine Citation:
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PMID: 22493080 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Inflammation alters hematopoiesis, often by decreasing erythropoiesis and enhancing myeloid output. The mechanisms behind these changes and how the BM stroma contributes to this process are active areas of research. In this study, we examine these questions in the setting of murine Toxoplasma gondii infection. Our data reveal that infection alters early myeloerythroid differentiation, blocking erythroid development beyond the Pre MegE stage, while expanding the GMP population. IL-6 was found to be a critical mediator of these differences, independent of hepcidin-induced iron restriction. Comparing the BM with the spleen showed that the hematopoietic response was driven by the local microenvironment, and BM chimeras demonstrated that radioresistant cells were the relevant source of IL-6 in vivo. Finally, direct ex vivo sorting revealed that VCAM(+)CD146(lo) BM stromal fibroblasts significantly increase IL-6 secretion after infection. These data suggest that BMSCs regulate the hematopoietic changes during inflammation via IL-6. |
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Authors:
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David B Chou; Brian Sworder; Nicolas Bouladoux; Cindy N Roy; Amiko M Uchida; Michael Grigg; Pamela G Robey; Yasmine Belkaid |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2012-04-09 |
Journal Detail:
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Title: Journal of leukocyte biology Volume: 92 ISSN: 1938-3673 ISO Abbreviation: J. Leukoc. Biol. Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-06-29 Completed Date: 2012-09-05 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 8405628 Medline TA: J Leukoc Biol Country: United States |
Other Details:
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Languages: eng Pagination: 123-31 Citation Subset: IM |
Affiliation:
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Mucosal Immunology, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Marrow / drug effects, parasitology, pathology Cell Differentiation / drug effects Cells, Cultured Colony-Forming Units Assay Erythroid Precursor Cells / drug effects*, parasitology, pathology Fibroblasts / cytology, drug effects, parasitology Hematopoiesis / drug effects Hematopoietic Stem Cells / drug effects, parasitology, pathology Interleukin-6 / pharmacology* Mice Mice, Inbred C57BL Mice, Knockout Myeloid Progenitor Cells / drug effects*, parasitology, pathology Stromal Cells / drug effects*, parasitology, pathology Toxoplasma / drug effects*, pathogenicity Toxoplasmosis / drug therapy*, parasitology, pathology |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK082722/DK/NIDDK NIH HHS; //Howard Hughes Medical Institute |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-6 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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