Document Detail

Stromal cell populations in necropsy bone marrow sections from allogeneic marrow recipients and non-transplant patients.
MedLine Citation:
PMID:  8157745     Owner:  NLM     Status:  MEDLINE    
AIMS: To compare the numbers of alkaline phosphatase positive reticulum cells (AL-RC) and macrophages in bone marrow transplant (BMT) recipients with numbers in normal subjects and to look for correlations with clinical features. METHODS: Sections of femoral marrow were obtained at necropsy from 18 BMT recipients and nine normal subjects who had died suddenly. AL-RC were visualised through their endogenous alkaline phosphatase activity. Macrophages were stained by an immunocytochemical technique using the antibody EBM/11 (CD68) and through their endogenous acid phosphatase activity. The numbers of stained cells were counted and expressed as a percentage of total nucleated cells. RESULTS: In both sets of marrow tissue, more macrophages stained for CD68 than for acid phosphatase, indicating macrophage heterogeneity. The percentage value for CD68 positive macrophages was higher among the transplant recipients (p < 0.01). At least in part this was caused by a reduction in haemopoietic cell numbers. Percentage values for acid phosphatase and alkaline phosphatase positive cells did not differ between the two groups. To exclude the effect of changes in marrow cellularity, stromal cell ratios were compared. The AL-RC: CD68 and acid phosphatase:CD68 ratios were both lower in BMT recipients, indicating that after BMT either the absolute number of AL-RC and acid phosphatase cells decreases, or CD68 cells increase, or there is a combination of the two. There was no correlation between the number of each cell type and cell dose given at transplantation, time after transplantation, presence of graft versus host disease or infection, marrow erythroid:myeloid ratio, or peripheral white cell count. The ratio of AL-RC to macrophages in our intact marrow was 0.43, considerably higher than that reported in cultured marrow. CONCLUSIONS: AL-RC and acid phosphatase positive cells may be most important for supporting haemopoiesis and their reduction after BMT may contribute to depression of haemopoiesis. CD68 positive cells include macrophages with a wide variety of functions and these may be increased in response to marrow damage.
S A Dilly; C J Jagger; J P Sloane
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical pathology     Volume:  46     ISSN:  0021-9746     ISO Abbreviation:  J. Clin. Pathol.     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1994-05-16     Completed Date:  1994-05-16     Revised Date:  2010-03-24    
Medline Journal Info:
Nlm Unique ID:  0376601     Medline TA:  J Clin Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  611-6     Citation Subset:  AIM; IM    
Department of Histopathology, St George's Hospital Medical School, London.
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MeSH Terms
Acid Phosphatase / analysis
Alkaline Phosphatase / analysis
Antigens, CD / analysis
Antigens, Differentiation, Myelomonocytic / analysis
Bone Marrow / pathology*
Bone Marrow Cells
Bone Marrow Transplantation / pathology*
Cell Count
Child, Preschool
Macrophages / chemistry,  cytology,  pathology
Middle Aged
Mononuclear Phagocyte System / cytology,  enzymology,  pathology
Stromal Cells / cytology,  pathology
Transplantation, Homologous
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, Differentiation, Myelomonocytic; 0/CD68 antigen, human; EC Phosphatase; EC Phosphatase

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