| Stroma-mediated granulocyte-macrophage colony-stimulating factor (GM-CSF) control of myelopoiesis: spatial organisation of intercellular interactions. | |
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MedLine Citation:
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PMID: 12827492 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Granulocyte-macrophage colony-stimulating factor (GM-CSF) is one of the major cytokines involved in control of haemopoiesis both in bone marrow and in extramedullar sites. Its biological activity depends upon the composition and physicochemical properties of the microenvironment provided by the supporting stroma. GM-CSF activity is modulated and controlled by the stromal heparan-sulphate proteoglycans, but their optimal interaction occurs only at low pH. We questioned whether the microenvironment organisation of the interface between stroma and haemopoietic cells provides such conditions. We studied myeloid progenitor proliferation in contact with bone marrow-derived and extramedullar stromas using electron microscopy and selective labelling of pericellular components. We present evidence that, upon interaction, the two cell types reorganise their interface both in shape and molecular composition. Haemopoietic cells extend projections that considerably increase the area of intercellular contact, and stromal cells form lamellipodia and carry out a redistribution of membrane-associated sialylated glycoconjugates and proteoglycans. Such rearrangements lead to extensive capping of negatively charged molecules at the interface between the supporting stroma and the haemopoietic cells, leading potentially to a local decrease in pH. Our results indicate that the distribution of negative charges at the cellular interface may be responsible for the selectivity of cell response to GM-CSF. |
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Authors:
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Radovan Borojevic; Marcelo Alex Carvalho; José Dias Corrêa-Junior; Katia Arcanjo; Laurecir Gomes; Paulo Pinto Joazeiro; Alex Balduino; Aline Wettreich; Tatiana Coelho-Sampaio |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2003-06-24 |
Journal Detail:
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Title: Cell and tissue research Volume: 313 ISSN: 0302-766X ISO Abbreviation: Cell Tissue Res. Publication Date: 2003 Jul |
Date Detail:
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Created Date: 2003-08-04 Completed Date: 2004-06-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0417625 Medline TA: Cell Tissue Res Country: Germany |
Other Details:
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Languages: eng Pagination: 55-62 Citation Subset: IM |
Affiliation:
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Departamento de Histologia-Embriologia, Instituto de Ciências Biomédicas, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, 21941-970, Rio de Janeiro, Brazil. radovan@iq.ufrj.br |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division / physiology Cell Line Cell Surface Extensions / ultrastructure Cells, Cultured Coculture Techniques Connective Tissue Cells / physiology Ferritins / analysis Fibroblasts / cytology, physiology Glycocalyx / chemistry, ultrastructure Glycosaminoglycans / analysis, isolation & purification, physiology Granulocyte-Macrophage Colony-Stimulating Factor / physiology* Hydrogen-Ion Concentration Indoles / analysis Mice Mice, Inbred C3H Microscopy, Electron Microscopy, Electron, Scanning Microscopy, Phase-Contrast Myeloid Progenitor Cells / chemistry, physiology, ultrastructure Myelopoiesis / physiology* N-Acetylneuraminic Acid / metabolism Neuraminidase / metabolism Organometallic Compounds / analysis Protein Binding Proteoglycans / analysis, physiology Pseudopodia / ultrastructure Ruthenium Red / analysis, pharmacology Scattering, Radiation Stromal Cells / chemistry, physiology, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Glycosaminoglycans; 0/Indoles; 0/Organometallic Compounds; 0/Proteoglycans; 0/polycationic ferritin; 11103-72-3/Ruthenium Red; 131-48-6/N-Acetylneuraminic Acid; 147-14-8/copper phthalocyanine; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor; 9007-73-2/Ferritins; EC 3.2.1.18/Neuraminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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