| Stretch and inflammation-induced Pre-B cell colony-enhancing factor (PBEF/Visfatin) and Interleukin-8 in amniotic epithelial cells. | |
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MedLine Citation:
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PMID: 20598369 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Preterm birth continues to be a growing problem in the USA. Although approximately half of preterm births are caused by intrauterine infection, uterine over-distension is also a cause. In this study we have compared the effects of static stretch, cyclic stretch/release and an inflammatory stimulus alone and in combination on the expression of Pre-B cell colony-enhancing factor (PBEF) and IL-8 in primary amniotic epithelial cells (AEC). We then sought to identify some of the mechanism(s) by which these cells respond to stretching stimuli. We show that cyclic stretch/release is a more robust stimulus for both PBEF and IL-8 than static stretch. Cyclic stretch/release increased both intracellular and secreted PBEF and a combination of both types of stretch was a more robust stimulus to PBEF that IL-8. However, when an inflammatory stimulus (IL-1beta) was added to either kind of stretch, the effect on IL-8 was much greater than that on PBEF. Thus, different kinds of stretch affect the expression of these two cytokines from AEC, but inflammation is a much stronger stimulus of IL-8 than PBEF, agreeing with its primary role as a chemokine. Although the AEC showed morphological signs of increased cellular stress during stretching, blocking reactive oxygen species (ROS) had little effect. However, blocking integrin binding to fibronectin significantly reduced the responses of both PBEF and IL-8 to cyclic stretch/release. The increased PBEF, both intracellularly and secreted, suggests that it functions both to increase the metabolism of the cells, at the same time as stimulating further the cytokine cascade leading to parturition. |
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Authors:
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C E Kendal-Wright; D Hubbard; J Gowin-Brown; G D Bryant-Greenwood |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-07-02 |
Journal Detail:
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Title: Placenta Volume: 31 ISSN: 1532-3102 ISO Abbreviation: Placenta Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-06 Completed Date: 2010-11-24 Revised Date: 2011-08-03 |
Medline Journal Info:
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Nlm Unique ID: 8006349 Medline TA: Placenta Country: England |
Other Details:
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Languages: eng Pagination: 665-74 Citation Subset: IM |
Affiliation:
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Department of Obstetrics, Gynecology and Women's Health, John A. Burns School of Medicine, University of Hawai'i, Honolulu, HI 96813, USA. kendal@pbrc.hawaii.edu |
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| MeSH Terms | |
Descriptor/Qualifier:
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Amnion
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metabolism* Cells, Cultured Cytokines / metabolism* Epithelial Cells / metabolism Female Gene Expression Humans Inflammation / metabolism* Integrins / metabolism Interleukin-8 / metabolism* Mechanotransduction, Cellular Nicotinamide Phosphoribosyltransferase / metabolism* Pregnancy Premature Birth / etiology Reactive Oxygen Species / metabolism Stress, Mechanical* Up-Regulation |
| Grant Support | |
ID/Acronym/Agency:
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U54 RR014607-1/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/IL8 protein, human; 0/Integrins; 0/Interleukin-8; 0/Reactive Oxygen Species; EC 2.4.2.12/Nicotinamide Phosphoribosyltransferase; EC 2.4.2.12/nicotinamide phosphoribosyltransferase, human |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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