Document Detail


Stress tolerance and transcriptional response in mouse embryos treated with high hydrostatic pressure to enhance cryotolerance.
MedLine Citation:
PMID:  21042655     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sublethal high hydrostatic pressure (HHP) treatment of cells was reported to enhance stress tolerance and to increase post-thawing survival after cryopreservation in mouse, swine and cattle. The goal of this study was to define if HHP stress tolerance depends on the embryos' stage of development and culture conditions, to describe long term in vivo effects and transcriptional alterations of selected stress related genes. Studies showed that impacts greater than 60 MPa caused blastomere and membrane injuries to the two-cell stage embryos, while even 80 MPa was well tolerated by blastocysts. HHP treatment caused significant upregulation of Azin1, Sod2 and Gadd45g genes, detected by RT-qPCR. The transfer of HHP treated blastocysts revealed normal in vivo development and reproductive function in a two generation study. The cell type and the embryos' development stage shall be taken into account when optimizing sublethal HHP stress treatment protocol of different cells.
Authors:
Istvan Bock; Eszter Losonczi; Solomon Mamo; Zsuzsanna Polgar; Andrea Harnos; Andras Dinnyes; Csaba Pribenszky
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryo letters     Volume:  31     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2010 Sep-Oct
Date Detail:
Created Date:  2010-11-02     Completed Date:  2010-12-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  England    
Other Details:
Languages:  eng     Pagination:  401-12     Citation Subset:  IM    
Affiliation:
Molecular Animal Biotechnology Laboratory, Institute for Basic Animal Sciences, Faculty of Agricultural and Environmental Sciences, Szent Istvan University, Godollo, Hungary. istvan.bock@biotalentum.hu
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological*
Animals
Blastocyst / cytology,  physiology
Cells, Cultured
Cold Temperature*
Cryopreservation*
Embryo, Mammalian / cytology*,  physiology
Hydrostatic Pressure*
Mice
Mice, Inbred ICR
Mice, Inbred Strains
Stress, Physiological / physiology
Transcription, Genetic*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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