Document Detail


Stress response of Escherichia coli to elevated hydrostatic pressure.
MedLine Citation:
PMID:  8226663     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The response of exponentially growing cultures of Escherichia coli to abrupt shifts in hydrostatic pressure was studied. A pressure upshift to 546 atm (55,304 kPa) of hydrostatic pressure profoundly perturbed cell division, nucleoid structure, and the total rate of protein synthesis. The number of polypeptides synthesized at increased pressure was greatly reduced, and many proteins exhibited elevated rates of synthesis relative to total protein synthesis. We designated the latter proteins pressure-induced proteins (PIPs). The PIP response was transient, with the largest induction occurring approximately 60 to 90 min postshift. Fifty-five PIPs were identified. Many of these proteins are also induced by heat shock or cold shock. The PIP demonstrating the greatest pressure induction was a basic protein of 15.6 kDa. High pressure inhibits growth but does not inhibit the synthesis of stringently controlled proteins. Cold shock is the only additional signal which has been found to elicit this type of response. These data indicate that elevated pressure induces a unique stress response in E. coli, the further characterization of which could be useful in delineating its inhibitory nature.
Authors:
T J Welch; A Farewell; F C Neidhardt; D H Bartlett
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  175     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1993-12-14     Completed Date:  1993-12-14     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7170-7     Citation Subset:  IM    
Affiliation:
Center for Marine Biomedicine and Biotechnology, Scripps Institution of Oceanography, University of California, San Diego, La Jolla 92093-0202.
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MeSH Terms
Descriptor/Qualifier:
Autoradiography
Bacterial Proteins / biosynthesis*,  isolation & purification
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Escherichia coli / cytology,  growth & development,  physiology*
Hydrostatic Pressure
Kinetics
Leucine / metabolism
Methionine / metabolism
Sulfur Radioisotopes
Time Factors
Tritium
Grant Support
ID/Acronym/Agency:
GM17892/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Sulfur Radioisotopes; 10028-17-8/Tritium; 61-90-5/Leucine; 63-68-3/Methionine
Comments/Corrections

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